Determination of HbA1c in the presence of haemoglobin variants:: Comparison of three HPLC techniques

被引：0
|
作者：
Carta, M [1 ]
Dall'Olio, G [1 ]
Soffiati, G [1 ]
机构：
[1] Osped S Bortolo, Chim Clin & Ematol Lab, I-36100 Vicenza, Italy
来源：
EUROPEAN JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY | 1997年 / 35卷 / 12期
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D O I：
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中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The presence of Hb variants may cause analytical interference in HbA(1c), values measured with the HPLC techniques currently used in Italian laboratories. In this study performance of a new HPLC system, (HA-8140, Menarini) was compared with two other traditional HPLC systems (HA-8121, Menarini and Diamat, Bio-Rad). The HA-8140 system detects the Kb variant possibly present in an independent peak. The integration area of such a peak is not computed in the calculation of the percentage value of HbA(1c). In this manner the underestimation of values obtained with traditional HPLC systems is avoided.
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页码：923 / 925
页数：3
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