Human limbal mesenchymal stem cells express ABCB5 and can grow on amniotic membrane

被引：18
|
作者：
Shaharuddin, Bakiah [1 ,2 ]
Osei-Bempong, Charles [1 ]
Ahmad, Sajjad [3 ,4 ]
Rooney, Paul [5 ]
Ali, Simi [6 ]
Oldershaw, Rachel [7 ]
Meeson, Annette [1 ]
机构：
[1] Newcastle Univ, Fac Med Sci, Inst Genet Med, Newcastle Upon Tyne NE1 3BZ, Tyne & Wear, England
[2] Univ Sains Malaysia, Adv Med & Dent Inst, George Town 13200, Malaysia
[3] Royal Liverpool Univ Hosp, St Pauls Eye Unit, Prescot St, Liverpool L7 8XP, Merseyside, England
[4] Univ Liverpool, Fac Hlth & Life Sci, Inst Ageing & Chron Dis, Dept Eye & Vis Sci, Liverpool L69 3GA, Merseyside, England
[5] NHS Blood & Transplant, Tissue Dev Lab, Estuary Banks, Liverpool L24 8RB, Merseyside, England
[6] Newcastle Univ, Inst Cellular Med, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
[7] Univ Liverpool, Dept Musculoskeletal Biol Grp 1, Inst Ageing & Chron Dis, Fac Hlth & Life Sci, Leahurst Campus,Chester High Rd, Neston CH64 7TE, Cheshire, England
来源：
REGENERATIVE MEDICINE | 2016年 / 11卷 / 03期
关键词：
ABCB5; amniotic membrane; limbal mesenchymal stem cells; limbal stem cells; limbal stem cell deficiency; SIDE POPULATION CELLS; EPITHELIAL-CELLS; GENE-EXPRESSION; STROMAL CELLS; TRANSPLANTATION; SURFACE; ABCG2; THERAPY; P63; DELTA-NP63;
D O I：
10.2217/rme-2016-0009
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
To isolate and characterize limbal mesenchymal stem cells (LMSCs) from human corneoscleral rings. Materials & methods: Cells were isolated from corneoscleral rings and cultured in a mesenchymal stem cell (MSC)-selective media and examined for differentiation, phenotyping and characterization. Results: LMSCs were capable of trilineage differentiation, adhered to tissue culture plastic, expressed HLA class I and cell surface antigens associated with human MSC while having no/low expression of HLA class II and negative hematopoietic lineage markers. They were capable for CXCL12-mediated cellular migration. LMSCs adhered, proliferated on amniotic membrane and expressed the common putative limbal stem cell markers. Conclusion: Limbal-derived MSC exhibited plasticity, could maintain limbal markers expression and demonstrated viable growth on amniotic membrane.
引用
收藏
页码：273 / 286
页数：14
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