Human limbal mesenchymal stem cells express ABCB5 and can grow on amniotic membrane

被引：18

作者：

Shaharuddin, Bakiah ^{[1
,2
]}

Osei-Bempong, Charles ^{[1
]}

Ahmad, Sajjad ^{[3
,4
]}

Rooney, Paul ^{[5
]}

Ali, Simi ^{[6
]}

Oldershaw, Rachel ^{[7
]}

Meeson, Annette ^{[1
]}

机构：

[1] Newcastle Univ, Fac Med Sci, Inst Genet Med, Newcastle Upon Tyne NE1 3BZ, Tyne & Wear, England

[2] Univ Sains Malaysia, Adv Med & Dent Inst, George Town 13200, Malaysia

[3] Royal Liverpool Univ Hosp, St Pauls Eye Unit, Prescot St, Liverpool L7 8XP, Merseyside, England

[4] Univ Liverpool, Fac Hlth & Life Sci, Inst Ageing & Chron Dis, Dept Eye & Vis Sci, Liverpool L69 3GA, Merseyside, England

[5] NHS Blood & Transplant, Tissue Dev Lab, Estuary Banks, Liverpool L24 8RB, Merseyside, England

[6] Newcastle Univ, Inst Cellular Med, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

[7] Univ Liverpool, Dept Musculoskeletal Biol Grp 1, Inst Ageing & Chron Dis, Fac Hlth & Life Sci, Leahurst Campus,Chester High Rd, Neston CH64 7TE, Cheshire, England

来源：

REGENERATIVE MEDICINE | 2016年 / 11卷 / 03期

关键词：

ABCB5; amniotic membrane; limbal mesenchymal stem cells; limbal stem cells; limbal stem cell deficiency; SIDE POPULATION CELLS; EPITHELIAL-CELLS; GENE-EXPRESSION; STROMAL CELLS; TRANSPLANTATION; SURFACE; ABCG2; THERAPY; P63; DELTA-NP63;

D O I：

10.2217/rme-2016-0009

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

To isolate and characterize limbal mesenchymal stem cells (LMSCs) from human corneoscleral rings. Materials & methods: Cells were isolated from corneoscleral rings and cultured in a mesenchymal stem cell (MSC)-selective media and examined for differentiation, phenotyping and characterization. Results: LMSCs were capable of trilineage differentiation, adhered to tissue culture plastic, expressed HLA class I and cell surface antigens associated with human MSC while having no/low expression of HLA class II and negative hematopoietic lineage markers. They were capable for CXCL12-mediated cellular migration. LMSCs adhered, proliferated on amniotic membrane and expressed the common putative limbal stem cell markers. Conclusion: Limbal-derived MSC exhibited plasticity, could maintain limbal markers expression and demonstrated viable growth on amniotic membrane.

引用

页码：273 / 286

页数：14

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