Spectrophotometric determination of lysozyme chloride in pharmaceutical preparations

被引：0
|
作者：
Naitoh, M [1 ]
Mori, I [1 ]
Matsuo, T [1 ]
Fujita, Y [1 ]
机构：
[1] Osaka Univ Pharmaceut Sci, Takatsuki, Osaka 56911, Japan
来源：
BUNSEKI KAGAKU | 1997年 / 46卷 / 12期
关键词：
lysozyme chloride determination by spectrophotometry; in pharmaceutical preparations; o-sulfophenylfluorone-titanium(IV) complex;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A spectrophotometric method for the determination of lysozyme chloride was established by using the o-sulfophenylfluorone (SPF)-titanium(IV) complex. This method can be used to determine about 10 similar to 200 mu g/10 ml lysozyme chloride, and is about 2-times more sensitive than the Japanese Standards of Pharmaceutical Ingredients method. This was applied to assays of lysozyme chloride in pharmaceutical preparations(tablet, granule, syrup, drysyrup, capsule and cold medicine). The analytical results and recoveries were satisfactory. The proposed method should be useful for the determination of various enzymes, such as alpha-amylase, cytochrome c and hyaluronidase.
引用
收藏
页码：1003 / 1007
页数：5
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