Spectrophotometric determination of lysozyme chloride in pharmaceutical preparations

A spectrophotometric method for the determination of lysozyme chloride was established by using the o-sulfophenylfluorone (SPF)-titanium(IV) complex. This method can be used to determine about 10 similar to 200 mu g/10 ml lysozyme chloride, and is about 2-times more sensitive than the Japanese Standards of Pharmaceutical Ingredients method. This was applied to assays of lysozyme chloride in pharmaceutical preparations(tablet, granule, syrup, drysyrup, capsule and cold medicine). The analytical results and recoveries were satisfactory. The proposed method should be useful for the determination of various enzymes, such as alpha-amylase, cytochrome c and hyaluronidase.