Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress

被引：7
|
作者：
Sun, Shao-kun [1 ,2 ]
Yang, Ni-na [1 ]
Chen, Li-jing [1 ,2 ]
Irfan, Muhammad [1 ]
Zhao, Xing-hua [3 ]
Li, Tian-lai [2 ]
机构：
[1] Shenyang Agr Univ, Coll Biol Sci & Technol, Key Lab Agr Biotechnol Liaoning Prov, Shenyang 110866, Liaoning, Peoples R China
[2] Shenyang Agr Univ, Minist Educ, Coll Hort, Key Lab Protected Hort, Shenyang 110866, Liaoning, Peoples R China
[3] Liaoning Acad Agr Sci, Shenyang 110161, Peoples R China
来源：
BIOMED RESEARCH INTERNATIONAL | 2015年 / 2015卷
基金：
中国博士后科学基金;
关键词：
GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE; CHILLING SENSITIVITY; MEMBRANE;
D O I：
10.1155/2015/792819
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity. Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.
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收藏
页数：8
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