Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress

被引：7

作者：

Sun, Shao-kun ^{[1
,2
]}

Yang, Ni-na ^{[1
]}

Chen, Li-jing ^{[1
,2
]}

Irfan, Muhammad ^{[1
]}

Zhao, Xing-hua ^{[3
]}

Li, Tian-lai ^{[2
]}

机构：

[1] Shenyang Agr Univ, Coll Biol Sci & Technol, Key Lab Agr Biotechnol Liaoning Prov, Shenyang 110866, Liaoning, Peoples R China

[2] Shenyang Agr Univ, Minist Educ, Coll Hort, Key Lab Protected Hort, Shenyang 110866, Liaoning, Peoples R China

[3] Liaoning Acad Agr Sci, Shenyang 110161, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2015年 / 2015卷

基金：

中国博士后科学基金;

关键词：

GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE; CHILLING SENSITIVITY; MEMBRANE;

D O I：

10.1155/2015/792819

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity. Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.

引用

页数：8

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