HPMC crosslinked chitosan/hydroxyapatite scaffolds containing Lemongrass oil for potential bone tissue engineering applications

The chitosan (CS), hydroxypropyl methyl cellulose (HPMC), hydroxyapatite (HAp and Lemon grass oil (LGO) based scaffolds was prepared by freeze gelation method. The composite for-mation was confirmed by FTIR (Fourier-transform infrared spectroscopy) analysis and surface morphology was evaluated by SEM (Scanning Electron Microscopy) analysis. The mechanical strength, biodegradation, swelling, porosity and antibacterial activity were evaluated on the basis of LGO contents. The scaffold structure was porous and the mechanical strength was enhanced as a function of LGO contents. The scaffold properties analysis revealed the biodegradation nature and swelling behavior of CS-HPMC-HAp-LGO was also affected significantly as a function of LGO contents. The cytotoxicity of CS-HPMC-HAp-LGO was studied against MC3T3-E1 cells and based on cell viability, no toxic sign was observed. The antimicrobial activity was evaluated against S. aureus and CS-HPMC-HAp-LGO scaffolds showed promising activity, which was varied as a function of LGO contents. The findings revealed that the CS-HPMC-HAp-LGO are biocom-patible and have potential for bone tissue engineering.(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).