Detection of Toxoplasma gondii by polymerase chain reaction in sera of acutely infected mice

被引:0
|
作者
Hafid, J [1 ]
Guichard, D [1 ]
Flori, P [1 ]
Bourlet, T [1 ]
Raberin, H [1 ]
Genin, C [1 ]
Sung, RTM [1 ]
机构
[1] Fac Med Jacques Lisfranc, GIMAP, F-42023 St Etienne 02, France
关键词
D O I
暂无
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 x 10(3) T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.
引用
收藏
页码:857 / 859
页数:3
相关论文
共 50 条
  • [21] DETECTION OF TOXOPLASMA-GONDII IN AQUEOUS-HUMOR BY THE POLYMERASE CHAIN-REACTION
    AOUIZERATE, F
    CAZENAVE, J
    POIRIER, L
    VERIN, P
    CHEYROU, A
    BEGUERET, J
    LAGOUTTE, F
    BRITISH JOURNAL OF OPHTHALMOLOGY, 1993, 77 (02) : 107 - 109
  • [22] Evaluation of quantitative polymerase chain reaction for the detection of Toxoplasma gondii oocysts shed by cats
    Miura, Ana Carolina
    de Barros, Luiz Daniel
    Minutti, Ana Flavia
    Martins, Thais Agostinho
    Sasse, Joao Pedro
    Lima Nino, Beatriz de Souza
    Garcia, Joao Luis
    REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, 2021, 30 (04):
  • [23] Differential detection of Hammondia hammondi from Toxoplasma gondii using polymerase chain reaction
    Sreekumar, C
    Vianna, MCB
    Hill, DE
    Miska, KB
    Lindquist, A
    Dubey, JP
    PARASITOLOGY INTERNATIONAL, 2005, 54 (04) : 267 - 269
  • [24] DETECTION OF TOXOPLASMA-GONDII WITH POLYMERASE CHAIN-REACTION FOR THE DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS
    KNERER, B
    HAYDE, M
    GRATZ, G
    BERNASCHEK, G
    STROBL, W
    POLLAK, A
    WIENER KLINISCHE WOCHENSCHRIFT, 1995, 107 (04) : 137 - 140
  • [25] Detection of Toxoplasma gondii in feline and canine biological samples by use of the polymerase chain reaction
    Stiles, J
    Prade, R
    Greene, C
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1996, 57 (03) : 264 - 267
  • [26] DETECTION OF CIRCULATING ANTIGENS IN SERA OF RABBITS INFECTED WITH TOXOPLASMA-GONDII
    ISE, Y
    IIDA, T
    SATO, K
    SUZUKI, T
    SHIMADA, K
    NISHIOKA, K
    INFECTION AND IMMUNITY, 1985, 48 (01) : 269 - 272
  • [27] A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction
    Pelloux, H
    Weiss, J
    Simon, J
    Muet, F
    FrickerHidalgo, H
    GoullierFleuret, A
    AmbroiseThomas, P
    FEMS MICROBIOLOGY LETTERS, 1996, 138 (01) : 11 - 15
  • [28] Development of a droplet digital polymerase chain reaction tool for the detection of Toxoplasma gondii in meat samples
    Andrea Mancusi
    Angela Giordano
    Antonio Bosco
    Santa Girardi
    Yolande T. R. Proroga
    Luigi Morena
    Renato Pinto
    Paolo Sarnelli
    Giuseppe Cringoli
    Laura Rinaldi
    Federico Capuano
    Maria Paola Maurelli
    Parasitology Research, 2022, 121 : 1467 - 1473
  • [29] Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction
    Kupferschmidt, O
    Krüger, D
    Held, TK
    Ellerbrok, H
    Siegert, W
    Janitschke, K
    CLINICAL MICROBIOLOGY AND INFECTION, 2001, 7 (03) : 120 - 124
  • [30] Polymerase chain reaction based detection of Toxoplasma gondii from lymph node aspirates of dogs
    Vijaya Bharathi M.
    Sreekumar C.
    Rajendran C.
    Journal of Parasitic Diseases, 2014, 38 (1) : 46 - 48