Detection of Toxoplasma gondii by polymerase chain reaction in sera of acutely infected mice

被引:0
|
作者
Hafid, J [1 ]
Guichard, D [1 ]
Flori, P [1 ]
Bourlet, T [1 ]
Raberin, H [1 ]
Genin, C [1 ]
Sung, RTM [1 ]
机构
[1] Fac Med Jacques Lisfranc, GIMAP, F-42023 St Etienne 02, France
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中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 x 10(3) T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.
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页码:857 / 859
页数:3
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