Effect of metformin/irinotecan-loaded poly-lactic-co-glycolic acid nanoparticles on glioblastoma: in vitro and in vivo studies

被引：46
|
作者：
Taghizadehghalehjoughi, Ali [1 ]
Hacimuftuoglu, Ahmet [2 ]
Cetin, Meltem [3 ]
Ugur, Afife Busra [3 ]
Galateanu, Bianca [4 ]
Mezhuev, Yaroslav [5 ]
Okkay, Ufuk [2 ]
Taspinar, Numan [2 ]
Taspinar, Mehmet [6 ]
Uyanik, Abdullah [7 ]
Gundogdu, Betul [8 ]
Mohammadzadeh, Maryam [2 ]
Nalci, Kemal Alp [2 ]
Stivaktakis, Polychronis [9 ,10 ]
Tsatsakis, Aristidis [9 ,10 ]
Jung, Tae Woo [11 ]
Jeong, Ji Hoon [12 ]
Abd El-Aty, A. M. [2 ,13 ]
机构：
[1] Ataturk Univ, Dept Pharmacol & Toxicol, Fac Vet Sci, TR-25240 Erzurum, Turkey
[2] Ataturk Univ, Dept Med Pharmacol, Fac Med, TR-25240 Erzurum, Turkey
[3] Ataturk Univ, Dept Pharmaceut Technol, Fac Pharm, TR-25240 Erzurum, Turkey
[4] Dept Biochem & Mol Biol, 91-95 Splaiul Independentei,Dist 5, Bucharest 050095, Romania
[5] D Mendeleev Univ Chem Technol Russia, Ctr Biomat, Miusskaya Sq 9, Moscow 125047, Russia
[6] Yuzuncu Yil Univ, Sch Med, Dept Med Biol, Van, Turkey
[7] Ataturk Univ, Dept Nephrol, Fac Med, TR-25240 Erzurum, Turkey
[8] Ataturk Univ, Dept Pathol, Fac Med, TR-25240 Erzurum, Turkey
[9] Univ Crete, Toxicol Lab, Sch Med, Iraklion 71003, Greece
[10] Univ Crete Spin Off, Toxplus SA, Iraklion 71601, Greece
[11] Seoul Natl Univ, Res Adm Team, Bundang Hosp, Gyeonggi, South Korea
[12] Chung Ang Univ, Dept Pharmacol, Coll Med, Seoul, South Korea
[13] Cairo Univ, Dept Pharmacol, Fac Vet Med, Giza 12211, Egypt
来源：
NANOMEDICINE | 2018年 / 13卷 / 13期
关键词：
brain tumor; Cavalieri's principle; irinotecan; metformin; nanoparticles; PLGA; IRINOTECAN CPT-11; METFORMIN; CANCER; GROWTH; CELL; THERAPY; GLIOMA; ZINGIBERENE; MULTIFORME; CARCINOMA;
D O I：
10.2217/nnm-2017-0386
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: The present study was designed to evaluate the effects of irinotecan hydrochloride (IRI)- or metformin hydrochloride (MET)-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for the treatment of glioblastoma multiforme using in vitro neuron and U-87 MG glioblastoma cell cultures and in vivo animal model. Methods: The cytotoxic and neurotoxic effects of pure drugs, blank NPs and MET- and IRI-loaded PLGA NPs were investigated in vitro (using methylthiazolyldiphenyl-tetrazolium bromide assay) and in vivo (using Cavalieri's principle for estimation of cancer volume). Results: 1 and 2 mM doses of MET and MET-loaded PLGA NPs, respectively, significantly reduced the volume of extracted cancer. Conclusion: Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach for the treatment of glioblastoma multiforme.
引用
收藏
页码：1595 / 1606
页数：12
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