A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish

被引：0
|
作者：
Liu, Ting-ting [1 ,2 ]
Hou, Han [1 ]
Du, Jiu-lin [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai Res Ctr Brain Sci & Brain Inspired Intell, Inst Neurosci,State Key Lab Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China
[2] Univ Chinese Acad Sci, 19A Yu Quan Rd, Beijing 100049, Peoples R China
[3] ShanghaiTech Univ, Sch Life Sci & Technol, 319 Yue Yang Rd, Shanghai 200031, Peoples R China
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 01期
关键词：
CALCIUM; DEPLETION; CURRENTS;
D O I：
10.1016/j.xpro.2021.100388
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca2+ activity and morphology of growing brain ETCs in larval zebrafish. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).
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页数：16
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