A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish

被引：0
|
作者：
Liu, Ting-ting [1 ,2 ]
Hou, Han [1 ]
Du, Jiu-lin [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai Res Ctr Brain Sci & Brain Inspired Intell, Inst Neurosci,State Key Lab Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China
[2] Univ Chinese Acad Sci, 19A Yu Quan Rd, Beijing 100049, Peoples R China
[3] ShanghaiTech Univ, Sch Life Sci & Technol, 319 Yue Yang Rd, Shanghai 200031, Peoples R China
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 01期
关键词：
CALCIUM; DEPLETION; CURRENTS;
D O I：
10.1016/j.xpro.2021.100388
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for understanding the mechanism of vascular development in organs, including the brain. Here, we describe a method for simultaneous monitoring and relevant analysis of the Ca2+ activity and morphology of growing brain ETCs in larval zebrafish. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).
引用
收藏
页数：16
相关论文
共 50 条
  • [31] Imaging Ca2+ activity in mammalian cells and zebrafish with a novel red-emitting aequorin variant
    Bakayan, Adil
    Domingo, Beatriz
    Miyawaki, Atsushi
    Llopis, Juan
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2015, 467 (09): : 2031 - 2042
  • [32] SIMULTANEOUS IMAGING OF INTRACELLULAR [CA2+] AND PH IN SINGLE MDCK AND GLOMERULAR EPITHELIAL-CELLS
    WIEGMANN, TB
    WELLING, LW
    BEATTY, DM
    HOWARD, DE
    VAMOS, S
    MORRIS, SJ
    AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (04): : C1184 - C1190
  • [33] A new technique for simultaneous and in situ measurements of Ca2+ signals in arteriolar smooth muscle and endothelial cells
    Burdyga, T
    Shmygol, A
    Eisner, DA
    Wray, S
    CELL CALCIUM, 2003, 34 (01) : 27 - 33
  • [34] Modulation of the effects of extracellular ATP on [Ca2+]i in rat brain microvascular endothelial cells
    Nobles, M
    Abbott, NJ
    EUROPEAN JOURNAL OF PHARMACOLOGY, 1998, 361 (01) : 119 - 127
  • [35] MODULATION OF INTRACELLULAR CA2+ CONCENTRATION IN BRAIN MICROVASCULAR ENDOTHELIAL CELLS IN VITRO BY ACOUSTIC CAVITATION
    Park, Juyoung
    Fan, Zhenzhen
    Kumon, Ronald E.
    El-Sayed, Mohamed E. H.
    Deng, Cheri X.
    ULTRASOUND IN MEDICINE AND BIOLOGY, 2010, 36 (07): : 1176 - 1187
  • [36] Novel mechanism for enhancement of Ca2+ response to ATP in bovine brain capillary endothelial cells
    Imaizumi, Y
    Yamazaki, D
    Aoyama, M
    Ohya, S
    Muraki, K
    Asai, K
    FASEB JOURNAL, 2005, 19 (04): : A517 - A517
  • [37] Estradiol effects on intracellular Ca2+ homeostasis in bovine brain-derived endothelial cells
    Matteo Suman
    Marta Giacomello
    Livio Corain
    Cristina Ballarin
    Stefano Montelli
    Bruno Cozzi
    Antonella Peruffo
    Cell and Tissue Research, 2012, 350 : 109 - 118
  • [38] Estradiol effects on intracellular Ca2+ homeostasis in bovine brain-derived endothelial cells
    Suman, Matteo
    Giacomello, Marta
    Corain, Livio
    Ballarin, Cristina
    Montelli, Stefano
    Cozzi, Bruno
    Peruffo, Antonella
    CELL AND TISSUE RESEARCH, 2012, 350 (01) : 109 - 118
  • [39] Tetrandrine inhibits Ca2+ release-activated Ca2+ channels in vascular endothelial cells
    Kwan, CY
    Leung, YM
    Kwan, TK
    Daniel, EE
    LIFE SCIENCES, 2001, 68 (07) : 841 - 847
  • [40] Capacitative Ca2+ entry is graded with depletion of intracellular Ca2+ stores in vascular endothelial cells
    Klishin, A
    Sedova, M
    Hüser, J
    Blatter, LA
    BIOPHYSICAL JOURNAL, 1999, 76 (01) : A225 - A225
12345