Identification of genetic evidence for Dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR

被引:39
|
作者
Weidmann, M
Schmidt, P
Vackova, A
Krivanec, K
Munclinger, P
Hufert, FT
机构
[1] Univ Hosp, Unit Microbiol Preparedness & Emerging Infect Dis, D-79014 Freiburg, Germany
[2] Purkyne Mil Med Acad, Dept Epidemiol, Hradec Kralove, Czech Republic
[3] Cent Mil Inst Hlth, Ceske Budejovice, Czech Republic
[4] Charles Univ, Biodivers Res Grp, Dept Zool, Prague, Czech Republic
关键词
D O I
10.1128/JCM.43.2.808-812.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A survey of 158 rodents caught in the Czech Republic identified Dobrava virus sequences closely related to that of the Dobrava virus type strain in Apodemus sylvaticus and Mus musculus rodents. The identity of A. sylvaticus was unequivocally confirmed by random amplified polymorphic DNA analysis. The data seem to indicate hantavirus spillover from Apodemus flavicollis to other rodents.
引用
收藏
页码:808 / 812
页数:5
相关论文
共 50 条
  • [31] Detection of Beet yellows virus by RT-PCR and immunocapture RT-PCR in Tetragonia expansa and Beta vulgaris
    Kundu, K
    Rysánek, P
    ACTA VIROLOGICA, 2004, 48 (03) : 177 - 182
  • [32] Diagnosis of Kyasanur forest disease by nested RT-PCR, real-time RT-PCR and IgM capture ELISA
    Mourya, Devendra T.
    Yadav, Pragya D.
    Mehla, Rajeev
    Barde, Pradip V.
    Yergolkar, Prasanna N.
    Kumar, Sandeep R. P.
    Thakare, Jyotsna P.
    Mishra, Akhilesh C.
    JOURNAL OF VIROLOGICAL METHODS, 2012, 186 (1-2) : 49 - 54
  • [33] Detection and quantification of WNV RNA with a TaqMan RT-PCR assay
    Tang, Y
    Hapip, CA
    Stramer, SL
    Fang, CT
    TRANSFUSION, 2003, 43 (09) : 3A - 3A
  • [34] TaqMan RT-PCR检测HAV方法的建立
    李晓婉
    陈琦
    金璘
    中外医疗, 2009, 28 (28) : 150 - 150
  • [35] Detection of infectious salmon anemia virus in sea water by nested RT-PCR
    Lovdal, T
    Enger, O
    DISEASES OF AQUATIC ORGANISMS, 2002, 49 (02) : 123 - 128
  • [36] A sensitive nested real-time RT-PCR for the detection of Shuni virus
    Van Eeden, C.
    Zaayman, D.
    Venter, M.
    JOURNAL OF VIROLOGICAL METHODS, 2014, 195 : 100 - 105
  • [37] Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR)
    Wang, GH
    Barton, C
    Rodgers, FG
    JOURNAL OF MICROBIOLOGICAL METHODS, 2002, 51 (01) : 119 - 121
  • [38] Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR
    Chandler, DP
    Wagnon, CA
    Bolton, H
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1998, 64 (02) : 669 - 677
  • [39] Rapid detection of infectious flacherie virus of the silkworm, Bombyx mori, using RT-PCR and nested PCR
    Vootla, Shyam Kumar
    Lu, Xing Meng
    Kari, Neetha
    Gadwala, Mallikarjun
    Lu, Qineng
    JOURNAL OF INSECT SCIENCE, 2013, 13
  • [40] Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus
    Zhou, Xinrong
    Zhang, Tiansheng
    Song, Deping
    Huang, Tao
    Peng, Qi
    Chen, Yanjun
    Li, Anqi
    Zhang, Fanfan
    Wu, Qiong
    Ye, Yu
    Tang, Yuxin
    MOLECULAR AND CELLULAR PROBES, 2017, 33 : 36 - 41