Identification of genetic evidence for Dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR

被引:39
|
作者
Weidmann, M
Schmidt, P
Vackova, A
Krivanec, K
Munclinger, P
Hufert, FT
机构
[1] Univ Hosp, Unit Microbiol Preparedness & Emerging Infect Dis, D-79014 Freiburg, Germany
[2] Purkyne Mil Med Acad, Dept Epidemiol, Hradec Kralove, Czech Republic
[3] Cent Mil Inst Hlth, Ceske Budejovice, Czech Republic
[4] Charles Univ, Biodivers Res Grp, Dept Zool, Prague, Czech Republic
关键词
D O I
10.1128/JCM.43.2.808-812.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A survey of 158 rodents caught in the Czech Republic identified Dobrava virus sequences closely related to that of the Dobrava virus type strain in Apodemus sylvaticus and Mus musculus rodents. The identity of A. sylvaticus was unequivocally confirmed by random amplified polymorphic DNA analysis. The data seem to indicate hantavirus spillover from Apodemus flavicollis to other rodents.
引用
收藏
页码:808 / 812
页数:5
相关论文
共 50 条
  • [21] Viremia in chronic hepatitis C patients evaluated by the Amplicor RT-PCR, a nested RT-PCR, and transaminase levels
    Laursen, AL
    Ingerslev, J
    Andersen, PL
    Ostergaard, L
    APMIS, 1998, 106 (02) : 334 - 338
  • [22] Evaluation of sensitivity of TaqMan RT-PCR for rubella virus detection in clinical specimens
    Okamoto, Kiyoko
    Mori, Yoshio
    Komagome, Rika
    Nagano, Hideki
    Miyoshi, Masahiro
    Okano, Motohiko
    Aoki, Yoko
    Ogura, Atsushi
    Hotta, Chiemi
    Ogawa, Tomoko
    Saikusa, Miwako
    Kodama, Hiroe
    Yasui, Yoshihiro
    Minagawa, Hiroko
    Kurata, Takako
    Kanbayashi, Daiki
    Kase, Tetsuo
    Murata, Sachiko
    Shirabe, Komei
    Hamasaki, Mitsuhiro
    Kato, Takashi
    Otsuki, Noriyuki
    Sakata, Masafumi
    Komase, Katsuhiro
    Takeda, Makoto
    JOURNAL OF CLINICAL VIROLOGY, 2016, 80 : 98 - 101
  • [23] Detection and identification of Fabavirus species by one-step RT-PCR and multiplex RT-PCR
    Panno, Stefano
    Ferriol, Inmaculada
    Rangel, Ezequiel A.
    Olmos, Antonio
    Han, Cheng-Gui
    Martinelli, Federico
    Rubio, Luis
    Davino, Salvatore
    JOURNAL OF VIROLOGICAL METHODS, 2014, 197 : 77 - 82
  • [24] Development of TaqMan RT-PCR assay for detection and quantification of dengue virus RNA
    Tang, Y.
    Liu, B.
    Hapip, C. A.
    Xu, D.
    Fang, C. T.
    TRANSFUSION, 2006, 46 (09) : 106A - 107A
  • [25] Design and validation of a universal reverse transcription PCR (RT-PCR) assay for vitiviruses infecting grapevine
    Diaz-Lara, A.
    Ericson, T. M.
    Al Rwahnih, M.
    Golino, D. A.
    PHYTOPATHOLOGY, 2020, 110 (12) : 45 - 45
  • [26] Integrated RT-PCR/nested PCR diagnosis for differentiating between subgroups of plum pox virus
    Szemes, M
    Kálmán, M
    Myrta, A
    Boscia, D
    Németh, M
    Kölber, M
    Dorgai, L
    JOURNAL OF VIROLOGICAL METHODS, 2001, 92 (02) : 165 - 175
  • [27] Detection of Citrus tatter leaf virus with reverse transcription—polymerase chain reaction (RT-PCR)
    D. L. Hailstones
    K. L. Bryant
    P. Broadbent
    C. Zhou
    Australasian Plant Pathology, 2000, 29 : 240 - 248
  • [28] Detection and identification of infectious bronchitis virus by RT-PCR in Iran
    Alireza Homayounimehr
    Ahmad Pakbin
    Reza Momayyez
    Seyyedeh Mahsa Rastegar Fatemi
    Tropical Animal Health and Production, 2016, 48 : 973 - 978
  • [29] Detection and identification of infectious bronchitis virus by RT-PCR in Iran
    Homayounimehr, Alireza
    Pakbin, Ahmad
    Momayyez, Reza
    Fatemi, Seyyedeh Mahsa Rastegar
    TROPICAL ANIMAL HEALTH AND PRODUCTION, 2016, 48 (05) : 973 - 978
  • [30] Comparison of low-density arrays, RT-PCR and real-time TaqMan® RT-PCR in detection of grapevine viruses
    Osman, Fatima
    Leutenegger, Christian
    Golino, Deborah
    Rowhani, Adib
    JOURNAL OF VIROLOGICAL METHODS, 2008, 149 (02) : 292 - 299