Identification of genetic evidence for Dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR

被引:39
|
作者
Weidmann, M
Schmidt, P
Vackova, A
Krivanec, K
Munclinger, P
Hufert, FT
机构
[1] Univ Hosp, Unit Microbiol Preparedness & Emerging Infect Dis, D-79014 Freiburg, Germany
[2] Purkyne Mil Med Acad, Dept Epidemiol, Hradec Kralove, Czech Republic
[3] Cent Mil Inst Hlth, Ceske Budejovice, Czech Republic
[4] Charles Univ, Biodivers Res Grp, Dept Zool, Prague, Czech Republic
关键词
D O I
10.1128/JCM.43.2.808-812.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A survey of 158 rodents caught in the Czech Republic identified Dobrava virus sequences closely related to that of the Dobrava virus type strain in Apodemus sylvaticus and Mus musculus rodents. The identity of A. sylvaticus was unequivocally confirmed by random amplified polymorphic DNA analysis. The data seem to indicate hantavirus spillover from Apodemus flavicollis to other rodents.
引用
收藏
页码:808 / 812
页数:5
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