Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae)

被引:15
|
作者
Guidugli, Marcela Corbo
Guerrieri Accoroni, Klaus Alvaro
Mestriner, Moacyr Antonio
Betioli Contel, Eucleia Primo
Martinez, Carlos Alberto [2 ]
Alzate-Marin, Ana Lilia [1 ]
机构
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Lab Genet Vegetal, BR-14049900 Ribeirao Preto, SP, Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Biol, BR-14049900 Ribeirao Preto, SP, Brazil
关键词
conservation; jequitiba rosa; population genetics; SSR markers; transferability; CROSS-SPECIES AMPLIFICATION; TRANSFERABILITY; POPULATION;
D O I
10.1590/S1415-47572010000100022
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guacu River basins in the state of Sao Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritance. The allelic richness at each locus ranged from 2-11, with an average of 7 alleles per locus, and the expected heterozygosity ranged from 0.07-0.88. These loci showed a high probability of paternity exclusion. The characteristics of these heterologous microsatellite markers indicate that they are suitable tools for investigating questions concerning population genetics in C. legalis.
引用
收藏
页码:131 / 134
页数:4
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