Imidazolium-tagged glycan probes for non-covalent labeling of live cells

被引：11

作者：

Benito-Alifonso, David ^{[1
]}

Tremell, Shirley ^{[1
]}

Sadler, Joanna C. ^{[1
]}

Berry, Monica ^{[2
]}

Galan, M. Carmen ^{[1
]}

机构：

[1] Univ Bristol, Sch Chem, Cantocks Close, Bristol BS8 1TS, Avon, England

[2] Univ Bristol, NSQI, Sch Phys, Tyndall Ave, Bristol BS8 1F, Avon, England

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 27期

基金：

英国工程与自然科学研究理事会; 欧洲研究理事会;

关键词：

SURFACE SIALIC ACIDS; CHEMICAL REPORTERS; OLIGOSACCHARIDE SYNTHESIS; CHEMOENZYMATIC SYNTHESIS; MODIFIED PROTEINS; CLICK CHEMISTRY; LIVING ANIMALS; IONIC LIQUIDS; GLYCOSYLATION; ISONITRILE;

D O I：

10.1039/c5cc10040b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.

引用

页码：4906 / 4909

页数：4

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