Two-Color Glycan Labeling of Live Cells by a Combination of DielsAlder and Click Chemistry

被引：161
|
作者：
Niederwieser, Andrea [1 ,2 ]
Spaete, Anne-Katrin [1 ,2 ]
Long Duc Nguyen [3 ]
Juengst, Christian [1 ,2 ]
Reutter, Werner [3 ]
Wittmann, Valentin [1 ,2 ]
机构：
[1] Univ Konstanz, Dept Chem, D-78457 Constance, Germany
[2] KoRS CB, D-78457 Constance, Germany
[3] Charite, CBF, Inst Biochem & Mol Biol, D-14195 Berlin, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 15期
关键词：
bioorthogonal reactions; carbohydrates; click chemistry; metabolic engineering; tetrazines; INVERSE-ELECTRON-DEMAND; AZIDE-ALKYNE CYCLOADDITION; GLCNAC-MODIFIED PROTEINS; ALDER REACTIONS; LIVING CELLS; IN-VIVO; BIOORTHOGONAL LIGATION; CHEMICAL REPORTERS; TRANS-CYCLOOCTENES; TERMINAL ALKYNES;
D O I：
10.1002/anie.201208991
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
One is not enough: Terminal alkenes are used as chemical reporters and ligation partners for 1,2,4,5-tetrazines in a Diels-Alder reaction with inverse electron demand (DARinv). Combination with strain-promoted azide-alkyne cycloaddition (SPAAC) allows the visualization of two different glycan structures in one experiment. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：4265 / 4268
页数：4
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