Microspore-derived embryo induction from cultured anthers of wheat

被引：7
|
作者：
Ingram, HM [1 ]
Power, JB [1 ]
Lowe, KC [1 ]
Davey, MR [1 ]
机构：
[1] Univ Nottingham, Sch Biol Sci, Plant Sci Div, Nottingham NG7 2RD, England
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2000年 / 60卷 / 03期
关键词：
anther culture; doubled haploids; microspore-derived embryos; phenylacetic acid; Poaceae; Triticum aestivum L. cv. Pavon;
D O I：
10.1023/A:1006400915551
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Microspore-derived embryo (MDE) induction was evaluated following the culture of wheat (Triticum aestivum L.) anthers in a basal medium with a range of supplements. Several supplements were evaluated, including a commercial bovine haemoglobin solution (Erythrogen(TM)), Ficoll and the co-polymer surfactant Pluronic F-68, but these did not enhance MDE induction. However, phenylacetic acid, replacing both 2,4-dichlorophenoxyacetic acid and kinetin, increased MDE induction by a factor of 1.6 (p < 0.05), producing 257 +/- 29 (mean +/- s.e., n=3) MDEs per 100 anthers cultured. Twenty-four plants regenerated from MDEs induced via anther culture were used for ploidy determinations; 33% were sterile haploids, while the remainder were fertile.
引用
收藏
页码：235 / 238
页数：4
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