Microspore-derived embryo induction from cultured anthers of wheat

被引：7

作者：

Ingram, HM ^{[1
]}

Power, JB ^{[1
]}

Lowe, KC ^{[1
]}

Davey, MR ^{[1
]}

机构：

[1] Univ Nottingham, Sch Biol Sci, Plant Sci Div, Nottingham NG7 2RD, England

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2000年 / 60卷 / 03期

关键词：

anther culture; doubled haploids; microspore-derived embryos; phenylacetic acid; Poaceae; Triticum aestivum L. cv. Pavon;

D O I：

10.1023/A:1006400915551

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microspore-derived embryo (MDE) induction was evaluated following the culture of wheat (Triticum aestivum L.) anthers in a basal medium with a range of supplements. Several supplements were evaluated, including a commercial bovine haemoglobin solution (Erythrogen(TM)), Ficoll and the co-polymer surfactant Pluronic F-68, but these did not enhance MDE induction. However, phenylacetic acid, replacing both 2,4-dichlorophenoxyacetic acid and kinetin, increased MDE induction by a factor of 1.6 (p < 0.05), producing 257 +/- 29 (mean +/- s.e., n=3) MDEs per 100 anthers cultured. Twenty-four plants regenerated from MDEs induced via anther culture were used for ploidy determinations; 33% were sterile haploids, while the remainder were fertile.

引用

页码：235 / 238

页数：4

共 50 条

[31] REGENERATION OF TRANSGENIC, MICROSPORE-DERIVED, FERTILE BARLEY
JAHNE, A
BECKER, D
BRETTSCHNEIDER, R
LORZ, H
THEORETICAL AND APPLIED GENETICS, 1994, 89 (04) : 525 - 533
[32] Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)
Hu, T
Kasha, KJ
CANADIAN JOURNAL OF PLANT SCIENCE, 1997, 77 (04) : 549 - 554
[33] Glutathione provides antioxidative defence and promotes microspore-derived embryo development in isolated microspore cultures of triticale (× Triticosecale Wittm.)
Iwona Żur
Ewa Dubas
Monika Krzewska
Kamil Zieliński
Jozsef Fodor
Franciszek Janowiak
Plant Cell Reports, 2019, 38 : 195 - 209
[34] Glutathione provides antioxidative defence and promotes microspore-derived embryo development in isolated microspore cultures of triticale (xTriticosecale Wittm.)
Zur, Iwona
Dubas, Ewa
Krzewska, Monika
Zielinski, Kamil
Fodor, Jozsef
Janowiak, Franciszek
PLANT CELL REPORTS, 2019, 38 (02) : 195 - 209
[35] Transformation of wheat (Triticum aestivum L.) microspore-derived callus and microspores by particle bombardment
Foiling L.
Olesen A.
Plant Cell Reports, 2001, 20 (07) : 629 - 636
[36] ARTIFICIAL SEEDS IN BARLEY - ENCAPSULATION OF MICROSPORE-DERIVED EMBRYOS
DATTA, SK
POTRYKUS, I
THEORETICAL AND APPLIED GENETICS, 1989, 77 (06) : 820 - 824
[37] Microspore-derived embryo formation in radish (Raphanus sativus L.) according to nutritional and environmental conditions
Changhoo Chun
Hanyong Park
Haeyoung Na
Horticulture, Environment, and Biotechnology, 2011, 52 : 530 - 535
[38] How microspores transform into haploid embryos:: changes associated with embryogenesis induction and microspore-derived embryogenesis
Segui-Simarro, Jose M.
Nuez, Fernando
PHYSIOLOGIA PLANTARUM, 2008, 134 (01) : 1 - 12
[39] IMPROVED EMBRYOID INDUCTION AND GREEN SHOOT REGENERATION FROM WHEAT ANTHERS CULTURED IN MEDIUM WITH MALTOSE
ORSHINSKY, BR
MCGREGOR, LJ
JOHNSON, GIE
HUCL, P
KARTHA, KK
PLANT CELL REPORTS, 1990, 9 (07) : 365 - 369
[40] NUCLEAR GAMETOPHYTIC GENES FROM CHROMOSOME ARM 1RS IMPROVE REGENERATION OF WHEAT MICROSPORE-DERIVED EMBRYOS
HENRY, Y
BERNARD, S
BERNARD, M
GAY, G
MARCOTTE, JL
DEBUYSER, J
GENOME, 1993, 36 (05) : 808 - 814

← 1 2 3 4 5 →