Development of an ic-ELISA and colloidal gold strip for the detection of the beta-blocker carazolol

被引：26
|
作者：
Zhou, Shengyang [1 ,2 ,3 ]
Kuang, Hua [1 ,2 ,3 ]
Liu, Liqiang [1 ,2 ,3 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China
[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Jiangsu, Peoples R China
[3] Jiangnan Univ, Sch Food Sci & Technol, Wuxi, Jiangsu, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2020年 / 31卷 / 01期
关键词：
Colloidal gold strip; CRZ; kidney; monoclonal antibody; milk; LINKED-IMMUNOSORBENT-ASSAY; RESONANCE RAYLEIGH-SCATTERING; LIQUID-CHROMATOGRAPHY; IMMUNOCHROMATOGRAPHIC ASSAY; CHLORTETRACYCLINE RESIDUES; BOVINE KIDNEY; TRANQUILIZERS; PORCINE; SEDATIVES; TISSUES;
D O I：
10.1080/09540105.2019.1710113
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A monoclonal antibody against carazolol (CRZ), 2F5, was generated for the construction of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip for detection of CRZ in milk and pig kidney samples. The antibody had a half-maximal inhibitory concentration of 0.29 ng/mL; its limit of detection was 0.068 ng/mL, and its linear range of detection was 0.137-0.638 ng/mL. For CRZ in milk samples and porcine kidney samples, the detection limits for this strip were 0.25 and 2.5 ng/mL, respectively. Analysis of CRZ in milk samples showed that the results obtained by immunochromatographic strip were consistent with those obtained by ic-ELISA. Therefore, the CG immunoassay is a sensitive screening method for semi-quantitative and qualitative detection of residual CRZ in milk and porcine kidney samples.
引用
收藏
页码：217 / 230
页数：14
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