Determination of 17α-methyltestosterone in Bovine Serum Using Liquid Chromatography Tandem Mass Spectrometry

被引：1

作者：

Regal, Patricia ^{[1
]}

Nebot, Carolina ^{[1
]}

Vazquez, Beatriz I. ^{[1
]}

Cepeda, Alberto ^{[1
]}

Fente, Cristina A. ^{[1
]}

机构：

[1] Univ Santiago de Compostela, Fac Vet, Area Nutr & Bromatol, Dept Quim Analit Nutr & Bromatol, Lugo 27002, Spain

来源：

JOURNAL OF FOOD AND DRUG ANALYSIS | 2010年 / 18卷 / 01期

关键词：

methyltestosterone; bovine serum; HPLC-MS/MS; validation; ANABOLIC-STEROIDS; DERIVATIZATION; IONIZATION; TESTOSTERONE; RESIDUES; HAIR; QUANTIFICATION; DERIVATIVES; ESTROGENS; TISSUE;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) based method has been developed and validated to confirm the presence of the synthetic growth promoter 17 alpha-methyltestosterone (MT) in bovine serum samples. After a single-step extraction with acetonitrile, the enhanced determination of methyltestosterone was achieved by detecting its oxime derivative. In accordance with the criteria defined in Commission Decision 2002/657/EC and using deuterated testosterone (T-d(3)) its an internal standard, the method has been validated in terms of decision limit (0.05 ng/mL), detection capability (0.09 ng/mL), repeatability CV (7.04%), within-laboratory reproducibility CV (11.00%), and accuracy (87.00%).

引用

页码：51 / 57

页数：7

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