Self-contained and modular structured illumination microscope

被引：4

作者：

Van den Eynde, Robin ^{[1
]}

Vandenberg, Wim ^{[1
]}

Hugelier, Siewert ^{[1
]}

Bouwens, Arno ^{[2
,3
]}

Hofkens, Johan ^{[2
,4
]}

Mueller, Marcel ^{[1
,5
]}

Dedecker, Peter ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Chem, Lab Nanobiol, Leuven, Belgium

[2] Katholieke Univ Leuven, Dept Chem, Mol Imaging & Photon, Leuven, Belgium

[3] Perseus Biom BV, Tienen, Belgium

[4] Max Planck Inst Polymer Res, Mainz, Germany

[5] Bielefeld Univ, Fac Phys, Bielefeld, Germany

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 07期

基金：

欧洲研究理事会;

关键词：

RESOLUTION; SPEED; LIMIT;

D O I：

10.1364/BOE.423492

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a modular implementation of structured illumination microscopy (SIM) that is fast, largely self-contained and that can be added onto existing fluorescence microscopes. Our strategy, which we call HIT-SIM, can theoretically deliver well over 50 super-resolved images per second and is readily compatible with existing acquisition software packages. We provide a full technical package consisting of schematics, a list of components and an alignment scheme that provides detailed specifications and assembly instructions. We illustrate the performance of the instrument by imaging optically large samples containing sequence-specifically stained DNA fragments. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：4414 / 4422

页数：9

共 50 条

[41] EXPERIMENTAL COMPUTATION OF SELF-CONTAINED STRESS
GRUTERS, H
MULLER, CH
ZEITSCHRIFT FUR ANGEWANDTE MATHEMATIK UND MECHANIK, 1979, 59 (05): : T184 - T186
[42] DATALOG - A SELF-CONTAINED TUTORIAL - COMMENT
KALINICHENKO, LA
PROGRAMMING AND COMPUTER SOFTWARE, 1991, 17 (04) : 212 - 213
[43] Self-Contained, Accurate Precomputation Prefetching
Atta, Islam
Tong, Xin
Srinivasan, Vijayalakshmi
Baldini, Loana
Moshovos, Andreas
PROCEEDINGS OF THE 48TH ANNUAL IEEE/ACM INTERNATIONAL SYMPOSIUM ON MICROARCHITECTURE (MICRO-48), 2015, : 153 - 165
[44] Smartphone Self-Contained NIR Colorimeter
Easin, Khaled Bin
Ifty, Mohiminur Rahman
Al Rayhan, Md. Sadik
Rani, Saptami
Maria, Nazmun Nahar
Hossain, Md. Arafat
Biswas, Protik Chandra
IEEE SENSORS LETTERS, 2024, 8 (12)
[45] SELF-CONTAINED CULTURE FLASK SYSTEM
PETERSON, JI
APPLIED MICROBIOLOGY, 1966, 14 (04) : 685 - &
[46] RAPID SELF-CONTAINED IMMUNOASSAY FOR DIGOXIN
BAHAR, I
VAUPEL, U
CASE, A
FARRELL, K
INBAR, S
METZMANN, E
CLINICAL CHEMISTRY, 1990, 36 (06) : 1028 - 1028
[47] OPEN SPACE VS SELF-CONTAINED
ARMSTRONG, DG
EDUCATIONAL LEADERSHIP, 1975, 32 (04) : 291 - 295
[48] A Laboratory Testbed for Self-Contained Navigation
Askari, Sina
Jao, Chi-Shih
Wang, Yusheng
Shkel, Andrei M.
2019 6TH IEEE INTERNATIONAL SYMPOSIUM ON INERTIAL SENSORS & SYSTEMS (INERTIAL 2019), 2019,
[49] Self-contained soft electrofluidic actuators
Tang, Wei
Lin, Yangqiao
Zhang, Chao
Liang, Yuwen
Wang, Jinrong
Wang, Wei
Ji, Chen
Zhou, Maoying
Yang, Huayong
Zou, Jun
SCIENCE ADVANCES, 2021, 7 (34)
[50] SELF-CONTAINED PORTABLE NOISE SHELTERS
不详
PLASTICS WORLD, 1979, 37 (06): : 74 - 74

← 1 2 3 4 5 →