Self-contained and modular structured illumination microscope

被引：4

作者：

Van den Eynde, Robin ^{[1
]}

Vandenberg, Wim ^{[1
]}

Hugelier, Siewert ^{[1
]}

Bouwens, Arno ^{[2
,3
]}

Hofkens, Johan ^{[2
,4
]}

Mueller, Marcel ^{[1
,5
]}

Dedecker, Peter ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Chem, Lab Nanobiol, Leuven, Belgium

[2] Katholieke Univ Leuven, Dept Chem, Mol Imaging & Photon, Leuven, Belgium

[3] Perseus Biom BV, Tienen, Belgium

[4] Max Planck Inst Polymer Res, Mainz, Germany

[5] Bielefeld Univ, Fac Phys, Bielefeld, Germany

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 07期

基金：

欧洲研究理事会;

关键词：

RESOLUTION; SPEED; LIMIT;

D O I：

10.1364/BOE.423492

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a modular implementation of structured illumination microscopy (SIM) that is fast, largely self-contained and that can be added onto existing fluorescence microscopes. Our strategy, which we call HIT-SIM, can theoretically deliver well over 50 super-resolved images per second and is readily compatible with existing acquisition software packages. We provide a full technical package consisting of schematics, a list of components and an alignment scheme that provides detailed specifications and assembly instructions. We illustrate the performance of the instrument by imaging optically large samples containing sequence-specifically stained DNA fragments. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：4414 / 4422

页数：9

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