Self-contained and modular structured illumination microscope

被引：4

作者：

Van den Eynde, Robin ^{[1
]}

Vandenberg, Wim ^{[1
]}

Hugelier, Siewert ^{[1
]}

Bouwens, Arno ^{[2
,3
]}

Hofkens, Johan ^{[2
,4
]}

Mueller, Marcel ^{[1
,5
]}

Dedecker, Peter ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Chem, Lab Nanobiol, Leuven, Belgium

[2] Katholieke Univ Leuven, Dept Chem, Mol Imaging & Photon, Leuven, Belgium

[3] Perseus Biom BV, Tienen, Belgium

[4] Max Planck Inst Polymer Res, Mainz, Germany

[5] Bielefeld Univ, Fac Phys, Bielefeld, Germany

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 07期

基金：

欧洲研究理事会;

关键词：

RESOLUTION; SPEED; LIMIT;

D O I：

10.1364/BOE.423492

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a modular implementation of structured illumination microscopy (SIM) that is fast, largely self-contained and that can be added onto existing fluorescence microscopes. Our strategy, which we call HIT-SIM, can theoretically deliver well over 50 super-resolved images per second and is readily compatible with existing acquisition software packages. We provide a full technical package consisting of schematics, a list of components and an alignment scheme that provides detailed specifications and assembly instructions. We illustrate the performance of the instrument by imaging optically large samples containing sequence-specifically stained DNA fragments. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：4414 / 4422

页数：9

共 50 条

[1] Modular membranes for self-contained reuse systems
Aya, Hidenori
Water Quality International, 1994, (04): : 21 - 22
[2] Self-Contained Modular Platform for Sensor in Electric Grids
Gevorkyan, V. M.
Kazantsev, Y. A.
Mikhalin, S. N.
Yashin, I. A.
2013 2ND MEDITERRANEAN CONFERENCE ON EMBEDDED COMPUTING (MECO), 2013,
[3] A Modular and Self-Contained Fluidic Engine for Soft Actuators
Bell, Michael A.
Gorissen, Benjamin
Bertoldi, Katia
Weaver, James C.
Wood, Robert J.
ADVANCED INTELLIGENT SYSTEMS, 2022, 4 (01)
[4] Self-Contained
Struthers, Ann
NORTH AMERICAN REVIEW, 2016, 301 (02): : 41 - 41
[5] A self-contained discipline?
Birch, AH
GOVERNMENT AND OPPOSITION, 2000, 35 (03) : 393 - 400
[6] THE SELF-CONTAINED CLASSROOM
WAGNER, G
EDUCATION, 1961, 82 (01): : 60 - 61
[7] America Self-Contained
Middlebush, Friderick A.
AMERICAN POLITICAL SCIENCE REVIEW, 1934, 28 (01) : 158 - 159
[8] Self-contained tensioning
Bleuvais, Sarah
Fougere, Edouard
ACTUALITES PHARMACEUTIQUES, 2020, 59 (600): : 11 - 11
[9] DEPARTMENTALIZED OR SELF-CONTAINED
SPIVAK, ML
PHI DELTA KAPPAN, 1957, 38 (04) : 134 - 135
[10] Self-contained laser
British Dental Journal, 2012, 212 (9) : 453 - 453

← 1 2 3 4 5 →