High-throughput protein sequencing

被引：11
|
作者：
Pham, V [1 ]
Tropea, J [1 ]
Wong, S [1 ]
Quach, J [1 ]
Henzel, WJ [1 ]
机构：
[1] Genentech Inc, Dept Prot Chem, San Francisco, CA 94080 USA
来源：
ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 04期
关键词：
D O I：
10.1021/ac0206317
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have designed and implemented an autosampler that provides additional sample capacity on a commercial protein sequencer. The autosampler attaches to a standard ABI Procise sequencer, enabling a single-sample cartridge to hold up to six separate samples. The autosampler is used in combination with faster Edman cycles and a rapid 12-min PTH separation to significantly increase the speed of automated protein sequencing. We also describe Edman chemistry modifications that improve coupling efficiency and prevent internal cleavage that can occur when samples are sequenced in the standard glass cartridges.
引用
收藏
页码：875 / 882
页数：8
相关论文
共 50 条
  • [41] High-throughput plasmid purification for capillary sequencing
    Elkin, CJ
    Richardson, PM
    Fourcade, HM
    Hammon, NM
    Pollard, MJ
    Predki, PF
    Glavina, T
    Hawkins, TL
    GENOME RESEARCH, 2001, 11 (07) : 1269 - 1274
  • [42] Diagnostic Applications of High-Throughput DNA Sequencing
    Boyd, Scott D.
    ANNUAL REVIEW OF PATHOLOGY: MECHANISMS OF DISEASE, VOL 8, 2013, 8 : 381 - 410
  • [43] High-throughput sequencing: what contribution to hematology?
    Labie, Dominique
    HEMATOLOGIE, 2014, 20 (01): : 66 - 67
  • [44] GeneProf: analysis of high-throughput sequencing experiments
    Florian Halbritter
    Harsh J Vaidya
    Simon R Tomlinson
    Nature Methods, 2012, 9 : 7 - 8
  • [45] Practical innovations for high-throughput amplicon sequencing
    Derek S Lundberg
    Scott Yourstone
    Piotr Mieczkowski
    Corbin D Jones
    Jeffery L Dangl
    Nature Methods, 2013, 10 : 999 - 1002
  • [46] Detecting and monitoring lymphoma with high-throughput sequencing
    Robins, Harlan
    ONCOTARGET, 2011, 2 (04) : 287 - 288
  • [47] DNA Fragment Enrichment for High-Throughput Sequencing
    A. N. Sinyakov
    E. V. Kostina
    Molecular Biology, 2023, 57 : 424 - 439
  • [48] High-throughput sequencing of microdissected chromosomal regions
    Weise, Anja
    Timmermann, Bernd
    Grabherr, Manfred
    Werber, Martin
    Heyn, Patricia
    Kosyakova, Nadezda
    Liehr, Thomas
    Neitzel, Heidemarie
    Konrat, Kateryna
    Bommer, Christiane
    Dietrich, Carola
    Rajab, Anna
    Reinhardt, Richard
    Mundlos, Stefan
    Lindner, Tom H.
    Hoffmann, Katrin
    EUROPEAN JOURNAL OF HUMAN GENETICS, 2010, 18 (04) : 457 - 462
  • [49] Nonoverlapping Clone Pooling for High-Throughput Sequencing
    Kuroshu, Reginaldo M.
    IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS, 2013, 10 (05) : 1091 - 1097
  • [50] High-throughput Sequencing Technology and Its Application
    Zhu Qiang-long
    Liu Shi
    Gao Peng
    Luan Fei-shi
    Journal of Northeast Agricultural University(English Edition), 2014, 21 (03) : 84 - 96
12345