High-throughput protein sequencing

被引：11
|
作者：
Pham, V [1 ]
Tropea, J [1 ]
Wong, S [1 ]
Quach, J [1 ]
Henzel, WJ [1 ]
机构：
[1] Genentech Inc, Dept Prot Chem, San Francisco, CA 94080 USA
来源：
ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 04期
关键词：
D O I：
10.1021/ac0206317
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have designed and implemented an autosampler that provides additional sample capacity on a commercial protein sequencer. The autosampler attaches to a standard ABI Procise sequencer, enabling a single-sample cartridge to hold up to six separate samples. The autosampler is used in combination with faster Edman cycles and a rapid 12-min PTH separation to significantly increase the speed of automated protein sequencing. We also describe Edman chemistry modifications that improve coupling efficiency and prevent internal cleavage that can occur when samples are sequenced in the standard glass cartridges.
引用
收藏
页码：875 / 882
页数：8
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