Charge switch derivatization of phosphopeptides for enhanced surface-enhanced Raman spectroscopy and mass spectrometry detection

被引：7
|
作者：
Li, Handong [1 ]
Sundararajan, Narayan [1 ]
机构：
[1] Intel Corp, Digital Hlth Grp, Santa Clara, CA 95054 USA
来源：
JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 08期
关键词：
phosphopeptides; charge switching; beta-elimination; michael addition; phosphonium or ammonium tagged peptides; surface-enhanced Raman spectroscopy (SERS); mass spectrometry;
D O I：
10.1021/pr0606225
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report an aqueous one-pot reaction chemistry to derivatize phosphopeptides by switching the negatively charged phosphate group to a positively charged phosphonium or ammonium moiety. The phosphonium or ammonium tagged peptides then serve as peptide or protein phosphorylation signatures allowing extended and more sensitive analyses using surface-enhanced Raman spectroscopy (SERS) and mass spectrometry.
引用
收藏
页码：2973 / 2977
页数：5
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