Automated, PCR-RFLP genotyping of the urokinase gene

被引：2
|
作者：
Sell, SM
Blasi, F
Booyse, F
机构：
[1] Univ Alabama, Dept Nutr Sci, Birmingham, AL 35294 USA
[2] Univ Alabama, Dept Med, Div Cardiovasc Dis, Birmingham, AL 35294 USA
[3] DIBIT HS Raffaele, Milan, Italy
来源：
GENETIC TESTING | 2000年 / 4卷 / 03期
关键词：
D O I：
10.1089/10906570050501551
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The urokinase-type plasminogen activator (u-PA) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease (CAD) (Grenett et al,, 1998). Recently, a common genetic polymorphism in the untranslated region of the Ic-PA gene was shown to be associated with syptomatic CAD. To study the possible role of this common genetic polymorphism in the Ic-PA gene, we have developed an automated, PCR-based assay. Automation of the PCR-RFLP genotyping of the BamHI polymorphism of the urokinase gene will support the screening of the large sample sizes required to do the population-based studies necessary to uncover disease susceptibility associations.
引用
收藏
页码：305 / 307
页数：3
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