Automated, PCR-RFLP genotyping of the urokinase gene

被引：2

作者：

Sell, SM

Blasi, F

Booyse, F

机构：

[1] Univ Alabama, Dept Nutr Sci, Birmingham, AL 35294 USA

[2] Univ Alabama, Dept Med, Div Cardiovasc Dis, Birmingham, AL 35294 USA

[3] DIBIT HS Raffaele, Milan, Italy

来源：

GENETIC TESTING | 2000年 / 4卷 / 03期

关键词：

D O I：

10.1089/10906570050501551

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The urokinase-type plasminogen activator (u-PA) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease (CAD) (Grenett et al,, 1998). Recently, a common genetic polymorphism in the untranslated region of the Ic-PA gene was shown to be associated with syptomatic CAD. To study the possible role of this common genetic polymorphism in the Ic-PA gene, we have developed an automated, PCR-based assay. Automation of the PCR-RFLP genotyping of the BamHI polymorphism of the urokinase gene will support the screening of the large sample sizes required to do the population-based studies necessary to uncover disease susceptibility associations.

引用

页码：305 / 307

页数：3

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