Biocompatibility Evaluation of Chitosan-based Injectable Hydrogels for the Culturing Mice Mesenchymal Stem Cells In Vitro

被引：61
|
作者：
Yan, Jihong [1 ,2 ]
Yang, Liu [1 ]
Wang, Guirong [1 ]
Xiao, Yang [1 ]
Zhang, Baohong [1 ]
Qi, Nianmin [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
[2] Capital Med Univ, Sch Basic Med Sci, Beijing 100069, Peoples R China
来源：
JOURNAL OF BIOMATERIALS APPLICATIONS | 2010年 / 24卷 / 07期
关键词：
chitosan; glycerophosphate salt; hydroxyethyl cellulose; thermosensitive injectable hydrogel; bone marrow mesenchymal stem cells; biocompatibility; BIODEGRADABLE POLYMERS; DELIVERY; GLYCEROPHOSPHATE; METHYLCELLULOSE; BIOMATERIALS; TEMPERATURE; SCAFFOLDS; BEHAVIOR; GELATION; BRAIN;
D O I：
10.1177/0885328208100536
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A thermosensitive polymer can be held liquid before being injected to deliver living cells or therapeutic agents and formed monolithic gels when injected in vivo. In this study, chitosan-based thermosensitive hydrogels were prepared, characterized and the biocompatibility for culturing mice bone mesenchymal stem cells (BMSC) on 2D films and within 3D hydrogel were investigated. The gelation temperature and biocompatibility could be modulated by addition hydroxyethyl cellulose (HEC) to chitosan-glycerophosphate (CH-GP) formulation. The CH- GP-HEC liquid solution can turn into gel at body temperature and has highly compatible with BMSC. Therefore, the CH- GP-HEC gel could be used as an attractive injected in-situ forming scaffold for future applications of delivering biologically active therapeutics for tissue engineering filed.
引用
收藏
页码：625 / 637
页数：13
相关论文
共 50 条
  • [41] Repair of osteochondral defects using injectable chitosan-based hydrogel encapsulated synovial fluid-derived mesenchymal stem cells in a rabbit model
    Jia, Zhaofeng
    Zhu, Feiyan
    Li, Xingfu
    Liang, Qian
    Zhuo, Zhenjian
    Huang, Jianghong
    Duan, Li
    Xiong, Jianyi
    Wang, Daping
    MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2019, 99 : 541 - 551
  • [42] Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity
    Rodriguez-Rodriguez, Rogelio
    Velasquillo-Martinez, Cristina
    Knauth, Peter
    Lopez, Zaira
    Moreno-Valtierra, Maira
    Bravo-Madrigal, Jorge
    Jimenez-Palomar, Ines
    Luna-Barcenas, Gabriel
    Espinosa-Andrews, Hugo
    Garcia-Carvajal, Zaira Y.
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2020, 108 (01) : 81 - 93
  • [43] Genipin-crosslinked chitosan hydrogels: Preliminary evaluation of the in vitro biocompatibility and biodegradation
    Vo, Nga T. N.
    Huang, Lei
    Lemos, Henrique
    Mellor, Andrew L.
    Novakovic, Katarina
    JOURNAL OF APPLIED POLYMER SCIENCE, 2021, 138 (34)
  • [44] Influence of umbilical cord mesenchymal stem cells culturing in vitro on their characteristics
    Deryabina, O. G.
    Maslova, O. O.
    Shuvalova, N. S.
    Sukhorada, O. M.
    Shpylova, S. P.
    Lykhacheva, L. I.
    Rymar, S. Y.
    Toporova, O. K.
    Kyryk, V. M.
    Makarenko, M. V.
    Govseiev, D. O.
    Kordium, V. A.
    HUMAN GENE THERAPY, 2012, 23 (10) : A64 - A64
  • [45] Tough and Cell-Compatible Chitosan Physical Hydrogels for Mouse Bone Mesenchymal Stem Cells in Vitro
    Ding, Beibei
    Gao, Huichang
    Song, Jianhui
    Li, Yaya
    Zhang, Lina
    Cao, Xiaodong
    Xu, Min
    Cai, Jie
    ACS APPLIED MATERIALS & INTERFACES, 2016, 8 (30) : 19739 - 19746
  • [46] Injectable chitosan-based hydrogels for drug delivery after ear-nose-throat surgery
    Schaffhausen, N.
    Tijsma, E.
    Hissong, B.
    JOURNAL OF CONTROLLED RELEASE, 2008, 132 (03) : E47 - E48
  • [47] Temperature- and pH-responsive chitosan-based injectable hydrogels for bone tissue engineering
    Lavanya, K.
    Chandran, S. Viji
    Balagangadharan, K.
    Selvamurugan, N.
    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2020, 111 (111):
  • [48] Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
    Li, Yongsan
    Zhang, Yaling
    Wei, Yen
    Tao, Lei
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2017, (127):
  • [49] Evaluation of the in vitro and in vivo biocompatibility of carrageenan-based hydrogels
    Popa, Elena G.
    Carvalho, Pedro P.
    Dias, Ana F.
    Santos, Tircia C.
    Santo, Vitor E.
    Marques, Alexandra P.
    Viegas, Carlos A.
    Dias, Isabel R.
    Gomes, Manuela E.
    Reis, Rui L.
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2014, 102 (11) : 4087 - 4097
  • [50] Chitosan-based interpolymeric pH-responsive hydrogels for in vitro drug release
    Abdelaal, M. Y.
    Abdel-Razik, E. A.
    Abdel-Bary, E. M.
    El-Sherbiny, I. M.
    JOURNAL OF APPLIED POLYMER SCIENCE, 2007, 103 (05) : 2864 - 2874
12345