Biocompatibility Evaluation of Chitosan-based Injectable Hydrogels for the Culturing Mice Mesenchymal Stem Cells In Vitro

被引：61
|
作者：
Yan, Jihong [1 ,2 ]
Yang, Liu [1 ]
Wang, Guirong [1 ]
Xiao, Yang [1 ]
Zhang, Baohong [1 ]
Qi, Nianmin [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
[2] Capital Med Univ, Sch Basic Med Sci, Beijing 100069, Peoples R China
来源：
JOURNAL OF BIOMATERIALS APPLICATIONS | 2010年 / 24卷 / 07期
关键词：
chitosan; glycerophosphate salt; hydroxyethyl cellulose; thermosensitive injectable hydrogel; bone marrow mesenchymal stem cells; biocompatibility; BIODEGRADABLE POLYMERS; DELIVERY; GLYCEROPHOSPHATE; METHYLCELLULOSE; BIOMATERIALS; TEMPERATURE; SCAFFOLDS; BEHAVIOR; GELATION; BRAIN;
D O I：
10.1177/0885328208100536
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A thermosensitive polymer can be held liquid before being injected to deliver living cells or therapeutic agents and formed monolithic gels when injected in vivo. In this study, chitosan-based thermosensitive hydrogels were prepared, characterized and the biocompatibility for culturing mice bone mesenchymal stem cells (BMSC) on 2D films and within 3D hydrogel were investigated. The gelation temperature and biocompatibility could be modulated by addition hydroxyethyl cellulose (HEC) to chitosan-glycerophosphate (CH-GP) formulation. The CH- GP-HEC liquid solution can turn into gel at body temperature and has highly compatible with BMSC. Therefore, the CH- GP-HEC gel could be used as an attractive injected in-situ forming scaffold for future applications of delivering biologically active therapeutics for tissue engineering filed.
引用
收藏
页码：625 / 637
页数：13
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