Apple fruit (Malus domestica Borkh. cv. Braeburn) were harvested during fruit development. The tissue was treated with phenol, acetic acid and water (PAW) to deactivate enzymes followed by alpha-amylase in H2O to remove starch. Water soluble polysaccharides were obtained from both fractions. The hemicelluloses were extracted from apple cell walls with 6 M NaOH. The carbohydrate composition of the cell wall fractions and the molecular weight of the hemicelluloses were determined. There was no change in the molecular weight profile of hemicellulose fractions or of xyloglucan polymers as obtained by HPLC with a size exclusion column. The cell wall changes in relation to cell expansion mechanisms are discussed. (C) 1997 Elsevier Science Ireland Ltd.
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Beijing Univ Chem Technol, Coll Int Educ, Beijing 100029, Peoples R ChinaBeijing Univ Chem Technol, Coll Int Educ, Beijing 100029, Peoples R China
Jia, Kenan
Wang, Wei
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China Agr Univ, Coll Hort, Beijing 100193, Peoples R ChinaBeijing Univ Chem Technol, Coll Int Educ, Beijing 100029, Peoples R China
Wang, Wei
Zhang, Qing
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Beijing Univ Agr, Coll Plant Sci & Technol, Key Lab Agr Applicat & New Tech, Beijing 102206, Peoples R ChinaBeijing Univ Chem Technol, Coll Int Educ, Beijing 100029, Peoples R China
Zhang, Qing
Jia, Wensuo
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China Agr Univ, Coll Hort, Beijing 100193, Peoples R China
Xinjiang Acad Agr Sci, Inst Hort Crops, Urumqi 830000, Xinjiang, Peoples R ChinaBeijing Univ Chem Technol, Coll Int Educ, Beijing 100029, Peoples R China