Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device

被引：12

作者：

Abe, Hiroya ^{[1
]}

Kanno, Yusuke ^{[1
]}

Ino, Kosuke ^{[1
]}

Inoue, Kumi Y. ^{[1
]}

Suda, Atsushi ^{[2
]}

Kunikata, Ryota ^{[2
]}

Matsudaira, Masahki ^{[3
]}

Shiku, Hitoshi ^{[1
]}

Matsue, Tomokazu ^{[1
,4
]}

机构：

[1] Tohoku Univ, Grad Sch Environm Studies, Aoba Ku, 6-6-11-604 Aramaki Aza Aoba, Sendai, Miyagi 9808579, Japan

[2] Japan Aviat Elect Ind Ltd, 1-1 Musashino 3 Chome, Tokyo 1968555, Japan

[3] Tohoku Univ, Micro Syst Integrat Ctr, Aoba Ku, 519-1176 Aramaki Aza Aoba, Sendai, Miyagi 9800845, Japan

[4] Tohoku Univ, WPI Adv Inst Mat Res, Aoba Ku, 2-1-1-509 Katahira, Sendai, Miyagi 9808577, Japan

来源：

ELECTROCHEMISTRY | 2016年 / 84卷 / 05期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Electrochemical Imaging; LSI-based Amperometric Device; Single-cell Analysis; Cell Adhesion; EMBRYOID BODIES; SENSOR ARRAY; CHIP DEVICE; RELEASE; MICROELECTRODES; EXOCYTOSIS; MICROSCOPY; ELECTRODES;

D O I：

10.5796/electrochemistry.84.364

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

We here report the electrochemical imaging of cell adhesion using a large-scale integration (LSI)-based amperometric device, called a Bio-LSI device. The device consists of 400 sensor electrodes arranged with a pitch of 250 pm. The device surface was modified with collagen to assist in the culture of MCF-7 cells and promote their adhesion. The cells disturb the electrochemical reaction of redox mediators, allowing the electrochemical signal to be used to evaluate cell adhesion at the single-cell level. This approach was applied to a cell detachment test. The results show that the Bio-LSI device is a promising tool for single-cell analysis. (C) The Electrochemical Society of Japan, All rights reserved.

引用

页码：364 / 367

页数：4

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