Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device

被引：12
|
作者：
Abe, Hiroya [1 ]
Kanno, Yusuke [1 ]
Ino, Kosuke [1 ]
Inoue, Kumi Y. [1 ]
Suda, Atsushi [2 ]
Kunikata, Ryota [2 ]
Matsudaira, Masahki [3 ]
Shiku, Hitoshi [1 ]
Matsue, Tomokazu [1 ,4 ]
机构：
[1] Tohoku Univ, Grad Sch Environm Studies, Aoba Ku, 6-6-11-604 Aramaki Aza Aoba, Sendai, Miyagi 9808579, Japan
[2] Japan Aviat Elect Ind Ltd, 1-1 Musashino 3 Chome, Tokyo 1968555, Japan
[3] Tohoku Univ, Micro Syst Integrat Ctr, Aoba Ku, 519-1176 Aramaki Aza Aoba, Sendai, Miyagi 9800845, Japan
[4] Tohoku Univ, WPI Adv Inst Mat Res, Aoba Ku, 2-1-1-509 Katahira, Sendai, Miyagi 9808577, Japan
来源：
ELECTROCHEMISTRY | 2016年 / 84卷 / 05期
基金：
日本科学技术振兴机构; 日本学术振兴会;
关键词：
Electrochemical Imaging; LSI-based Amperometric Device; Single-cell Analysis; Cell Adhesion; EMBRYOID BODIES; SENSOR ARRAY; CHIP DEVICE; RELEASE; MICROELECTRODES; EXOCYTOSIS; MICROSCOPY; ELECTRODES;
D O I：
10.5796/electrochemistry.84.364
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
We here report the electrochemical imaging of cell adhesion using a large-scale integration (LSI)-based amperometric device, called a Bio-LSI device. The device consists of 400 sensor electrodes arranged with a pitch of 250 pm. The device surface was modified with collagen to assist in the culture of MCF-7 cells and promote their adhesion. The cells disturb the electrochemical reaction of redox mediators, allowing the electrochemical signal to be used to evaluate cell adhesion at the single-cell level. This approach was applied to a cell detachment test. The results show that the Bio-LSI device is a promising tool for single-cell analysis. (C) The Electrochemical Society of Japan, All rights reserved.
引用
收藏
页码：364 / 367
页数：4
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