Plant regeneration from callus culture of a Paphiopedilum hybrid

被引：57

作者：

Lin, YH ^{[1
]}

Chang, C ^{[1
]}

Chang, WC ^{[1
]}

机构：

[1] Acad Sinica, Inst Bot, Taipei 115, Taiwan

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2000年 / 62卷 / 01期

关键词：

callus proliferation; Paphiopedilum; plant regeneration; protocorm formation; totipotent callus;

D O I：

10.1023/A:1006425214471

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Totipotent calli of a Paphiopedilum hybrid ( Paphiopedilum callosum 'Oakhi' x Paph. lawrenceanum 'Tradition') were induced from seed-derived protocorms on a 1/2 strength Murashige-Skoog medium plus 1-10 mg l(-1) 2,4- dichlorophenoxyacetic acid (2,4-D) and 0.1-1 mg l(-1) 1- phenyl-3-(1.2.3-thiadiazol-5-yl)urea (TDZ). These calli grew well when subcultured on the same medium, but proliferated more on 1/2 MS medium plus 5 mg l(-1) 2,4-D and 1 mg l(-1) TDZ. Calli developed further along a route of production of protocorm-like bodies and eventually formed plantlets that could be transplanted to pots and grew well.

引用

页码：21 / 25

页数：5

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