Plant regeneration from callus culture of a Paphiopedilum hybrid

Totipotent calli of a Paphiopedilum hybrid ( Paphiopedilum callosum 'Oakhi' x Paph. lawrenceanum 'Tradition') were induced from seed-derived protocorms on a 1/2 strength Murashige-Skoog medium plus 1-10 mg l(-1) 2,4- dichlorophenoxyacetic acid (2,4-D) and 0.1-1 mg l(-1) 1- phenyl-3-(1.2.3-thiadiazol-5-yl)urea (TDZ). These calli grew well when subcultured on the same medium, but proliferated more on 1/2 MS medium plus 5 mg l(-1) 2,4-D and 1 mg l(-1) TDZ. Calli developed further along a route of production of protocorm-like bodies and eventually formed plantlets that could be transplanted to pots and grew well.