Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

被引：15
|
作者：
Wu, Jiang-Chong [2 ,3 ]
Yang, Jing [2 ,3 ]
Gu, Zhi-Jian [3 ]
Zhang, Yan-Ping [1 ]
机构：
[1] Chinese Acad Forestry, Res Inst Resource Insects, Kunming 650224, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
[3] Chinese Acad Sci, Kunming Bot Garden, Kunming Inst Bot, Kunming 650204, Peoples R China
来源：
HORTSCIENCE | 2010年 / 45卷 / 04期
关键词：
Horse radish tree; drumstick tree; SSR makers; population structure; population genetics; GENETIC-VARIATION; AFLP;
D O I：
10.21273/HORTSCI.45.4.690
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.
引用
收藏
页码：690 / 692
页数：3
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