In vitro Characterization of PBLG-g-HA/PLLA Nanocomposite Scaffolds

被引：6
|
作者：
Liao Lan [1 ,2 ]
Yang Shuang [3 ,4 ]
Miron, Richard J. [3 ,4 ]
Wei Junchao [1 ]
Zhang Yufeng [3 ,4 ,5 ]
Zhang Meng [1 ]
机构：
[1] Nanchang Univ, Sch Mat Sci & Engn, Nanchang 330031, Peoples R China
[2] Nanchang Univ, Sch Stomatol, Nanchang 330006, Peoples R China
[3] Wuhan Univ, State Key Lab Breeding Base Basic Sci Stomatol Hu, Sch & Hosp Stomatol, Wuhan 430079, Peoples R China
[4] Wuhan Univ, Key Lab Oral Biomed, Minist Educ, Sch & Hosp Stomatol, Wuhan 430079, Peoples R China
[5] Wuhan Univ, Sch Stomatol, Dept Oral Implantol, Wuhan 430079, Peoples R China
来源：
JOURNAL OF WUHAN UNIVERSITY OF TECHNOLOGY-MATERIALS SCIENCE EDITION | 2014年 / 29卷 / 04期
基金：
中国国家自然科学基金;
关键词：
hydroxyapatite; poly(L-lactide); tissue engineering; biomaterials; MARROW STROMAL CELLS; GROWTH-FACTORS; HYDROXYAPATITE; POLYLACTIDE; DIFFERENTIATION; RELEASE; VIVO;
D O I：
10.1007/s11595-014-1006-4
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The purpose of the present study was to synthesize a new composites scaffold containing poly(gamma-benzyl-L-glutamate) modified hydroxyapatite/(poly (L-lactic acid)) (PBLG-g-HA/PLLA) and to investigate their in vitro behaviour on bone mesenchymal stromal cells (BMSCs). The results demonstrated that BMSC proliferation was significantly increased on PBLG-g-HA/PLLA scaffolds after 3 and 7 days post seeding when compared to PLLA and HA/PLLA scaffolds. The in vitro osteogenic differentiation also favoured the composite PBLG-g-HA/PLLA scaffolds when compared to controls by significantly increasing Runx2, ALP or osteocalcin mRNA expression as assessed by real-time PCR. The results illustrate the potential of PBLG-g-HA/PLLA scaffolds for bone tissue engineering applications. And the in vivo testing further confirms the PBLG-g-HA/PLLA scaffolds' potentioal for healing critical bone defects.
引用
收藏
页码：841 / 847
页数：7
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