Functional Elucidation and Methylation-Mediated Downregulation of ITGA5 Gene in Breast Cancer Cell Line MDA-MB-468

被引：23
|
作者：
Fang, Zhengyu [1 ,2 ]
Yao, Wantong [3 ]
Xiong, Yi [1 ,2 ]
Zhang, Jufeng [1 ,2 ]
Liu, Li [1 ,2 ]
Li, Jiana [1 ,2 ]
Zhang, Chao [1 ,2 ]
Wan, Jun [1 ,2 ]
机构：
[1] Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen, Guangdong, Peoples R China
[2] Peking Univ, Shenzhen Hosp, Shenzhen, Guangdong, Peoples R China
[3] Fudan Univ, Shanghai Med Coll, Dept Biochem & Mol Biol, Shanghai 200032, Peoples R China
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2010年 / 110卷 / 05期
基金：
中国博士后科学基金;
关键词：
ITGA5; BREAST CANCER; CELL-ECM INTERACTION; CELL PROLIFERATION; MIGRATION; INTEGRIN EXPRESSION; MALIGNANT-MELANOMA; LIGAND-BINDING; FIBRONECTIN; ADHESION; GROWTH; METASTASIS; ALPHA-5; TRANSDUCTION; HETERODIMER;
D O I：
10.1002/jcb.22626
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Expression level of integrin alpha 5 in tumor cells has been indicated to be involved in cell proliferation and organ-specific metastasis We previously demonstrated that ITGA5 expression was downregulated in the high invasive MDA-MB-468 cells compared with other breast cancer cell lines. In this study, we found that the methylation status in the region around transcriptional start site of ITGA5 gene was increased in MDA-MB-468 cells Overexpression of integrin alpha 5 on MDA-MB-468 cells resulted in cell growth inhibition, which could be reversed by adhesion to fibronectin Cell adhesion and spreading to fibronectin was enhanced after ITGA5 was overexpressed in MDA-MB-468 cells, while cell migration was attenuated. Knockdown of ITGA5 in MCF-7 cells led to cell growth inhibition but had little influence on cell migration These findings indicated the diverse roles of ITGA5 expression in breast cancer cells J Cell Biochem 110 1130-1141, 2010 (C) 2010 Wiley-Liss. Inc
引用
收藏
页码：1130 / 1141
页数：12
相关论文
共 50 条
  • [31] KNOCKDOWN OF TRIM66 IN MDA-MB-468 TRIPLE NEGATIVE BREAST CANCER CELL LINE SUPPRESSES PROLIFERATION AND PROMOTES APOPTOSIS THROUGH EGFR SIGNALING
    Zhang, Huayi
    Zheng, Yaqin
    Zhang, Yongmei
    POLISH JOURNAL OF PATHOLOGY, 2021, 72 (02) : 160 - 166
  • [32] Solubility enhancement of morin and epicatechin through encapsulation in an albumin based nanoparticulate system and their anticancer activity against the MDA-MB-468 breast cancer cell line
    Ghosh, Pooja
    Bag, Sudipta
    Roy, Atanu Singha
    Subramani, Elavarasan
    Chaudhury, Koel
    Dasgupta, Swagata
    RSC ADVANCES, 2016, 6 (103) : 101415 - 101429
  • [33] A new platinum complex of triazine demonstrates G1 arrest with novel biological profile in human breast cancer cell line, MDA-MB-468
    Mandal, Soma
    Berube, Gervais
    Asselin, Eric
    Richardson, Vernon J.
    Church, Jon G.
    Bridson, John
    Pham, Tram N. Q.
    Pramanik, Saroj K.
    Mandal, Sanat K.
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2007, 17 (08) : 2139 - 2145
  • [34] Antibacterial Synthetic Peptides Derived from Bovine Lactoferricin Exhibit Cytotoxic Effect against MDA-MB-468 and MDA-MB-231 Breast Cancer Cell Lines
    Casanova, Yerly Vargas
    Rodriguez Guerra, Jorge Antonio
    Umana Perez, Yadi Adriana
    Leal Castro, Aura Lucia
    Reina, Giovanni Almanzar
    Garcia Castaneda, Javier Eduardo
    Rivera Monroy, Zuly Jenny
    MOLECULES, 2017, 22 (10):
  • [35] Mechanism of inhibition of MDA-MB-468 breast cancer cell growth by 2,3,7,8-tetrachlorodibenzo-p-dioxin
    Wang, WL
    Porter, W
    Burghardt, R
    Safe, SH
    CARCINOGENESIS, 1997, 18 (05) : 925 - 933
  • [36] Defective repair of 8-hydroxyguanine in mitochondria of MCF-7 and MDA-MB-468 human breast cancer cell lines
    Mambo, E
    Nyaga, SG
    Bohr, VA
    Evans, MK
    CANCER RESEARCH, 2002, 62 (05) : 1349 - 1355
  • [37] SAR optimization studies on a novel series of 2-anilinopyrimidines as selective inhibitors against triple-negative breast cancer cell line MDA-MB-468
    Jo, Jeyun
    Kim, Heegyu
    Oh, Ji Youn
    Kim, Soyeong
    Park, Yeong Hye
    Choi, Hyeonjin
    Jeong, Jee-Yeong
    Jung, Young-Suk
    Yun, Hwayoung
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2019, 29 (24)
  • [38] Discovery and SAR studies of novel 2-anilinopyrimidine-based selective inhibitors against triple-negative breast cancer cell line MDA-MB-468
    Jo, Jeyun
    Kim, Sou Hyun
    Kim, Heegyu
    Jeong, Myeonggyo
    Kwak, Jae-Hwan
    Han, Young Taek
    Jeong, Jee-Yeong
    Jung, Young-Suk
    Yun, Hwayoung
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2019, 29 (01) : 62 - 65
  • [39] Antiproliferative and cytostatic effects of the natural product eupatorin on MDA-MB-468 human breast cancer cells due to CYP1-mediated metabolism
    Vasilis Androutsopoulos
    Randolph RJ Arroo
    John F Hall
    Somchaiya Surichan
    Gerry A Potter
    Breast Cancer Research, 10
  • [40] INDUCTION OF CELL APOPTOSIS BY BILIVERDIN REDUCTASE INHIBITOR IN MCF-7 AND MDA-MB-468 BREAST CANCER CELL LINES: EXPERIMENTAL AND IN SILICO STUDIES
    Shahrokhi, Seyedeh Zahra
    Tehrani, Fatemeh Soghra Karami
    Salami, Siamak
    EXCLI JOURNAL, 2021, 20 : 1502 - 1516
12345