Functional Elucidation and Methylation-Mediated Downregulation of ITGA5 Gene in Breast Cancer Cell Line MDA-MB-468

被引：23
|
作者：
Fang, Zhengyu [1 ,2 ]
Yao, Wantong [3 ]
Xiong, Yi [1 ,2 ]
Zhang, Jufeng [1 ,2 ]
Liu, Li [1 ,2 ]
Li, Jiana [1 ,2 ]
Zhang, Chao [1 ,2 ]
Wan, Jun [1 ,2 ]
机构：
[1] Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen, Guangdong, Peoples R China
[2] Peking Univ, Shenzhen Hosp, Shenzhen, Guangdong, Peoples R China
[3] Fudan Univ, Shanghai Med Coll, Dept Biochem & Mol Biol, Shanghai 200032, Peoples R China
来源：
JOURNAL OF CELLULAR BIOCHEMISTRY | 2010年 / 110卷 / 05期
基金：
中国博士后科学基金;
关键词：
ITGA5; BREAST CANCER; CELL-ECM INTERACTION; CELL PROLIFERATION; MIGRATION; INTEGRIN EXPRESSION; MALIGNANT-MELANOMA; LIGAND-BINDING; FIBRONECTIN; ADHESION; GROWTH; METASTASIS; ALPHA-5; TRANSDUCTION; HETERODIMER;
D O I：
10.1002/jcb.22626
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Expression level of integrin alpha 5 in tumor cells has been indicated to be involved in cell proliferation and organ-specific metastasis We previously demonstrated that ITGA5 expression was downregulated in the high invasive MDA-MB-468 cells compared with other breast cancer cell lines. In this study, we found that the methylation status in the region around transcriptional start site of ITGA5 gene was increased in MDA-MB-468 cells Overexpression of integrin alpha 5 on MDA-MB-468 cells resulted in cell growth inhibition, which could be reversed by adhesion to fibronectin Cell adhesion and spreading to fibronectin was enhanced after ITGA5 was overexpressed in MDA-MB-468 cells, while cell migration was attenuated. Knockdown of ITGA5 in MCF-7 cells led to cell growth inhibition but had little influence on cell migration These findings indicated the diverse roles of ITGA5 expression in breast cancer cells J Cell Biochem 110 1130-1141, 2010 (C) 2010 Wiley-Liss. Inc
引用
收藏
页码：1130 / 1141
页数：12
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