Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

被引：58
|
作者：
Melles, Damian C.
van Leeuwen, Willem B.
Snijders, Susan V.
Horst-Kreft, Deborah
Peeters, Justine K.
Verbrugh, Henri A.
van Belkum, Alex
机构：
[1] Univ Med Ctr Rotterdam, Erasmus MC, Dept Med Microbiol & Infect Dis, NL-3015 GD Rotterdam, Netherlands
[2] Univ Med Ctr Rotterdam, Erasmus MC, Dept Bioinformat, NL-3000 DR Rotterdam, Netherlands
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 69卷 / 02期
关键词：
Staphylococcus aureus; molecular typing; MLST; PFGE; AFLP;
D O I：
10.1016/j.mimet.2007.01.013
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We compared multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for typing of Staphylococcus aureus and show that the methods yield similar results, although with differences in resolving power and reproducibility. Epidemiological conditions should determine which is the optimal typing method to be used. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：371 / 375
页数：5
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