Comparison of multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for genetic typing of Staphylococcus aureus

被引：58
|
作者：
Melles, Damian C.
van Leeuwen, Willem B.
Snijders, Susan V.
Horst-Kreft, Deborah
Peeters, Justine K.
Verbrugh, Henri A.
van Belkum, Alex
机构：
[1] Univ Med Ctr Rotterdam, Erasmus MC, Dept Med Microbiol & Infect Dis, NL-3015 GD Rotterdam, Netherlands
[2] Univ Med Ctr Rotterdam, Erasmus MC, Dept Bioinformat, NL-3000 DR Rotterdam, Netherlands
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 69卷 / 02期
关键词：
Staphylococcus aureus; molecular typing; MLST; PFGE; AFLP;
D O I：
10.1016/j.mimet.2007.01.013
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We compared multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) for typing of Staphylococcus aureus and show that the methods yield similar results, although with differences in resolving power and reproducibility. Epidemiological conditions should determine which is the optimal typing method to be used. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：371 / 375
页数：5
相关论文
共 50 条
  • [21] Multilocus sequence typing lacks the discriminatory ability of pulsed-field gel electrophoresis for typing Salmonella enterica serovar Typhimurium
    Fakhr, MK
    Nolan, LK
    Logue, CM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (05) : 2215 - 2219
  • [22] Klebsiella typing:: pulsed-field gel electrophoresis (PFGE) in comparison with O:K-serotyping
    Hansen, DS
    Skov, R
    Benedí, JV
    Sperling, V
    Kolmos, HJ
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2002, 8 (07) : 397 - 404
  • [23] Assessment of Candida glabrata strain relatedness by pulsed-field gel electrophoresis and multilocus sequence typing
    Lin, Chi-Yang
    Chen, Yee-Chun
    Lo, Hsiu-Jung
    Chen, Kuo-Wei
    Li, Shu-Ying
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (08) : 2452 - 2459
  • [24] TYPING OF STAPHYLOCOCCUS-AUREUS BY PULSED-FIELD GEL-ELECTROPHORESIS, ZYMOTYPING, CAPSULAR TYPING, AND PHAGE TYPING - RESOLUTION OF CLONAL RELATIONSHIPS
    SCHLICHTING, C
    BRANGER, C
    FOURNIER, JM
    WITTE, W
    BOUTONNIER, A
    WOLZ, C
    GOULLET, P
    DORING, G
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (02) : 227 - 232
  • [25] Comparison of direct genome Restriction Enzyme analysis and pulsed-field gel electrophoresis for typing of Vibrio vulnificus and their correspondence with multilocus sequence typing data
    Gonzalez-Escalona, Narjol
    Whitney, Brooke
    Jaykus, Lee-Ann
    DePaola, Angelo
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (22) : 7494 - 7500
  • [26] Epidemiological typing of methicillin-resistant Staphylococcus aureus (MRSA): spa typing versus pulsed-field gel electrophoresis
    Melin, Sara
    Haeggman, Sara
    Olsson-Liljequist, Barbro
    Sjolund, Maria
    Nilsson, Peter A.
    Isaksson, Barbro
    Lofgren, Sture
    Matussek, Andreas
    [J]. SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 2009, 41 (6-7) : 433 - 439
  • [27] Comparison of seven techniques for typing international epidemic strains of Clostfidium difficile:: Restriction endonuclease analysis, pulsed-field gel electrophoresis, PCR-ribotyping, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, amplified fragment length polymorphism, and surface layer protein A gene sequence typing
    Killgore, George
    Thompson, Angela
    Johnson, Stuart
    Brazier, Jon
    Kuijper, Ed
    Pepin, Jacques
    Frost, Eric H.
    Savelkoul, Paul
    Nicholson, Brad
    van den Berg, Renate J.
    Kato, Haru
    Sambol, Susan P.
    Zukowski, Walter
    Woods, Christopher
    Limbago, Brandi
    Gerding, Dale N.
    McDonald, L. Clifford
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (02) : 431 - 437
  • [28] Inter-centre comparison of pulsed-field gel electrophoresis for the typing of methicillin-resistant Staphylococcus aureus
    Cookson, BD
    Aparicio, P
    Deplano, A
    Struelens, M
    Goering, R
    Marples, R
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 1996, 44 (03) : 179 - 184
  • [29] PULSED-FIELD GEL-ELECTROPHORESIS AS A REPLACEMENT FOR BACTERIOPHAGE-TYPING OF STAPHYLOCOCCUS-AUREUS
    BANNERMAN, TL
    HANCOCK, GA
    TENOVER, FC
    MILLER, JM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) : 551 - 555
  • [30] Genetic diversity in Italian Lactobacillus sanfranciscensis strains assessed by multilocus sequence typing and pulsed-field gel electrophoresis analyses
    Picozzi, Claudia
    Bonacina, Gaia
    Vigentini, Ileana
    Foschino, Roberto
    [J]. MICROBIOLOGY-SGM, 2010, 156 : 2035 - 2045
12345