Determining the genetic structure of the natural population of Staphylococcus aureus:: A comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing

被引：92
|
作者：
Grundmann, H [1 ]
Hori, S
Enright, MC
Webster, C
Tami, A
Feil, EJ
Pitt, T
机构：
[1] Univ Nottingham Hosp, Queens Med Ctr, Div Microbiol & Infect Dis, Nottingham NG7 2UH, England
[2] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
[3] Cent Publ Hlth Lab, Div Nosocomial Infect Prevent & Control, Lab Hosp Infect, London NW9 5HT, England
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 12期
关键词：
D O I：
10.1128/JCM.40.12.4544-4546.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We used a sample of Staphylococcus aureus strains that are carried by humans and that are representative of the natural population of S. aureus strains in order to assess the value of multilocus sequence typing (MLST), pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing as epidemiological tools. Only MLST was able to define clonal complexes unambiguously. All DNA-based typing approaches achieved a high degree of agreement, implying phylogenetic concordance, but predicted epidemiological associations with variable accuracy.
引用
收藏
页码：4544 / 4546
页数：3
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