Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata

被引：33
|
作者：
Pan, XW [1 ]
Xu, HH [1 ]
Liu, X [1 ]
Gao, X [1 ]
Lu, YT [1 ]
机构：
[1] Wuhan Univ, Coll Life Sci, Key Lab MOE Plant Dev Biol, Wuhan 430072, Hubei Province, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 22期
关键词：
ammonium; Camptotheca acuminata; camptothecin; nitrate; two-stage culture;
D O I：
10.1007/s10529-004-4580-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Nitrate at 70 mM gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3- molar ratio of 5:1 (giving a total of 40 mM N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l(-1), 0.36 mg g(-1), and 12.8 mg l(-1) , respectively.
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页码：1745 / 1748
页数：4
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