Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata

被引：33

作者：

Pan, XW ^{[1
]}

Xu, HH ^{[1
]}

Liu, X ^{[1
]}

Gao, X ^{[1
]}

Lu, YT ^{[1
]}

机构：

[1] Wuhan Univ, Coll Life Sci, Key Lab MOE Plant Dev Biol, Wuhan 430072, Hubei Province, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 22期

关键词：

ammonium; Camptotheca acuminata; camptothecin; nitrate; two-stage culture;

D O I：

10.1007/s10529-004-4580-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nitrate at 70 mM gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3- molar ratio of 5:1 (giving a total of 40 mM N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l(-1), 0.36 mg g(-1), and 12.8 mg l(-1) , respectively.

引用

页码：1745 / 1748

页数：4

共 46 条

[21] Yield improvement in plant cell suspension cultures by in-situ extraction
Pedersen, H
Dutta, A
Chin, CK
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1997, 213 : 23 - AGFD
[22] Effect of nitrogen source on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng
Zhang, YH
Zhong, JJ
Yu, JT
BIOTECHNOLOGY PROGRESS, 1996, 12 (04) : 567 - 571
[23] EFFECT OF SOURCE OF INORGANIC NITROGEN ON GROWTH AND ENZYMES OF NITROGEN ASSIMILATION IN SOYBEAN AND WHEAT CELLS IN SUSPENSION CULTURES
BAYLEY, JM
KING, J
GAMBORG, OL
PLANTA, 1972, 105 (01) : 15 - &
[24] Artemisinin production in Artemisia annua hairy root cultures with improved growth by altering the nitrogen source in the medium
Wang, JW
Tan, RX
BIOTECHNOLOGY LETTERS, 2002, 24 (14) : 1153 - 1156
[25] Artemisinin production in Artemisia annua hairy root cultures with improved growth by altering the nitrogen source in the medium
Jian Wen Wang
Ren Xiang Tan
Biotechnology Letters, 2002, 24 : 1153 - 1156
[26] Soybean genetic improvement in yield and the effect of late-season shading and nitrogen source and supply
Kumudini, S.
Omielan, J.
Hume, D. J.
AGRONOMY JOURNAL, 2008, 100 (02) : 400 - 405
[27] EFFECTS OF NITROGEN-SOURCE ON BETACYANIN ACCUMULATION AND GROWTH IN SUSPENSION-CULTURES OF PHYTOLACCA-AMERICANA
SAKUTA, M
TAKAGI, T
KOMAMINE, A
PHYSIOLOGIA PLANTARUM, 1987, 71 (04) : 459 - 463
[28] GROWTH OF PLANT-CELL SUSPENSION-CULTURES ON GLYCEROL AS A SOLE SOURCE OF CARBON AND ENERGY
JONES, A
VELIKY, IA
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1980, 58 (06): : 648 - 657
[29] GROWTH AND EMBRYO FORMATION IN WILD-CARROT SUSPENSION CULTURES WITH AMMONIUM ION AS A SOLE NITROGEN-SOURCE
DOUGALL, DK
VERMA, DC
IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION, 1978, 14 (02): : 180 - 182
[30] FACTORS RELATED TO GROWTH AND NITROGEN ASSIMILATION IN CELL SUSPENSION CULTURES OF DOUGLAS-FIR (Pseudotsuga menziesii)
Lee, Moon Soo
Kirby, Edward G.
PLANT PHYSIOLOGY, 1983, 72 : 47 - 47

← 1 2 3 4 5 →