Biolistic® transformation of cacao [Theobroma cacao (L.)]

被引：0
|
作者：
Perry, MD [1 ]
Power, JB [1 ]
Lowe, KC [1 ]
Davey, MR [1 ]
机构：
[1] Univ Nottingham, Sch Biol Sci, Plant Sci Div, Nottingham NG7 2RD, England
来源：
TROPICAL AGRICULTURE | 2000年 / 77卷 / 01期
关键词：
microprojectile bombardment; woody plant; Theobroma cacao;
D O I：
暂无
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Callus of cacao [Theobroma cacao (L.)] were transformed by particle bombardment (Biolistics(R)). Four-week-old floral-derived calli were bombarded with plasmid containing the beta -glucuronidase (gus) gene, driven by a CaMV 35S promoter. Histochemical analysis of gus gene expression was used to optimize the parameters for deoxyribonucleic acid (DNA) delivery. More than 270 histochemically positive (blue staining) transformation events were observed with staminode-derived calli bombarded at 1550 psi.
引用
收藏
页码：64 / 66
页数：3
相关论文
共 50 条
  • [1] Regionalization of Cacao (Theobroma cacao L.) in Colombia
    Gonzalez-Orozco, Carlos E.
    Pesca, Allende
    [J]. FRONTIERS IN SUSTAINABLE FOOD SYSTEMS, 2022, 6
  • [2] The Criollo cacao tree (Theobroma cacao L.): a review
    Lachenaud, P.
    Motamayor, J. C.
    [J]. GENETIC RESOURCES AND CROP EVOLUTION, 2017, 64 (08) : 1807 - 1820
  • [3] The Criollo cacao tree (Theobroma cacao L.): a review
    P. Lachenaud
    J. C. Motamayor
    [J]. Genetic Resources and Crop Evolution, 2017, 64 : 1807 - 1820
  • [4] Morphological and genetic diversity of cacao (Theobroma cacao L.) in Uganda
    David Gopaulchan
    Lambert A. Motilal
    Frances L. Bekele
    Séverine Clause
    James O. Ariko
    Harriet P. Ejang
    Pathmanathan Umaharan
    [J]. Physiology and Molecular Biology of Plants, 2019, 25 : 361 - 375
  • [5] The Effect of Cacao (Theobroma cacao L.) Pulp on Final Flavour
    Ali, N. A.
    Baccus-Taylor, G. S. H.
    Sukha, D. A.
    Umaharan, P.
    [J]. III INTERNATIONAL CONFERENCE ON POSTHARVEST AND QUALITY MANAGEMENT OF HORTICULTURAL PRODUCTS OF INTEREST FOR TROPICAL REGIONS, 2014, 1047 : 245 - 254
  • [6] Morphological and genetic diversity of cacao (Theobroma cacao L.) in Uganda
    Gopaulchan, David
    Motilal, Lambert A.
    Bekele, Frances L.
    Clause, Severine
    Ariko, James O.
    Ejang, Harriet P.
    Umaharan, Pathmanathan
    [J]. PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 2019, 25 (02) : 361 - 375
  • [7] Phylogenetic diversity of cacao (Theobroma cacao L.) genotypes in Colombia
    Gonzalez-Orozco, Carlos E.
    Osorio-Guarin, Jaime A.
    Yockteng, Roxana
    [J]. PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION, 2022, 20 (03): : 194 - 202
  • [8] Aroma precursors in Cacao (Theobroma cacao L.) Chabasquen Portuguesa
    Alvarado, M.
    Portillo, E.
    Boulanger, R.
    Bastide, P.
    Macia, I.
    [J]. REVISTA DE LA FACULTAD DE AGRONOMIA DE LA UNIVERSIDAD DEL ZULIA, 2014, 31 : 812 - 823
  • [9] ANATOMY AND HISTOCHEMISTRY OF THE MEXICAN CACAO (Theobroma cacao L.) SEED
    Rangel-Fajardo, Ma Alma
    Zavaleta-Mancera, Hilda A.
    Cordova-Tellez, Leobigildo
    Lopez-Andrade, Alejandro P.
    Delgado-Alvarado, Adriana
    Vidales-Fernandez, Ignacio
    Villegas-Monter, Angel
    [J]. REVISTA FITOTECNIA MEXICANA, 2012, 35 (03) : 189 - 197
  • [10] Cadmium Prevalence in Cacao (Theobroma cacao L.) and Potential Remediation Strategies
    Hussain, Babar
    Liu, Shibin
    Rahman, Shafeeq Ur
    Leiva-Espinoza, Santos Triunfo
    Javed, Khadija
    Pu, Shengyan
    Farooq, Muhammad
    [J]. JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, 2023, 23 (03) : 2938 - 2954
12345