TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells

被引：4
|
作者：
Yan, Ying [1 ,2 ]
Liu, Feng [3 ]
Dang, Xitong [4 ]
Zhou, Rui [4 ]
Liao, Bin [3 ]
机构：
[1] Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610075, Sichuan, Peoples R China
[2] Southwest Med Univ, Coll Integrated Tradit Chinese & Western Med, Luzhou 646000, Sichuan, Peoples R China
[3] Southwest Med Univ, Dept Cardiac Macrovasc Surg, Affiliated Hosp, 3-319 Zhongshan Rd, Luzhou 646000, Sichuan, Peoples R China
[4] Southwest Med Univ, Key Lab Med Electrophysiol, Collaborat Innovat Ctr Prevent & Treatment Cardio, Minist Educ & Med Electrophysiol,Inst Cardiovasc, Luzhou 646000, Sichuan, Peoples R China
来源：
GENE EXPRESSION PATTERNS | 2021年 / 40卷
关键词：
Cardiomyocytes; Human induced pluripotent stem cells (hiPSC); T-box transcription factor 3 (TBX3); Sinoatrial node like cells (SANLC); CONDUCTION SYSTEM; SHOX2; GENE; SINOATRIAL; MOUSE;
D O I：
10.1016/j.gep.2021.119184
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored. Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC), TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SANspecific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5. Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells.
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页数：8
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