If this study, 809 samples of ice cream from different sources were investigated by using cultural methods for the presence of presumptive Bacillus cereus. Isolates front culture-positive samples were examined with a real-time PCR assay targeting a region of the cereulide synthetase gene (ces) that is highly specific for emetic B. cereus strains. The samples were collected front ice cream parlors and restaurants that produced their own ice cream and from international commercial ice cream companies in different regions of Bavaria during the summer of 2008. Presumptive B. cereus was found in 508 (62.7%) ice cream samples investigated, and 24 (4.7%) of the isolates had the genetic background for cereulide toxin production. The level of emetic B. cereus in the positive samples ranged from 0.1 to 20 CFU/g of ice cream.
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Korea Res Inst, Dept Food Safety & Distribut Res Grp, Wonju 55365, Jeollabuk Do, South KoreaKorea Res Inst, Dept Food Safety & Distribut Res Grp, Wonju 55365, Jeollabuk Do, South Korea
Park, Kyung Min
Kim, Hyun Jung
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Korea Res Inst, Dept Food Safety & Distribut Res Grp, Wonju 55365, Jeollabuk Do, South Korea
Korea Univ Sci & Technol, Dept Food Biotechnol, Daejeon 34113, South KoreaKorea Res Inst, Dept Food Safety & Distribut Res Grp, Wonju 55365, Jeollabuk Do, South Korea
Kim, Hyun Jung
Park, Kee Jai
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Korea Res Inst, Dept Food Safety & Distribut Res Grp, Wonju 55365, Jeollabuk Do, South KoreaKorea Res Inst, Dept Food Safety & Distribut Res Grp, Wonju 55365, Jeollabuk Do, South Korea
Park, Kee Jai
Koo, Minseon
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Korea Res Inst, Dept Food Safety & Distribut Res Grp, Wonju 55365, Jeollabuk Do, South Korea
Korea Univ Sci & Technol, Dept Food Biotechnol, Daejeon 34113, South KoreaKorea Res Inst, Dept Food Safety & Distribut Res Grp, Wonju 55365, Jeollabuk Do, South Korea