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Simultaneous enrichment and separation of flavonoids from Herba Epimedii by macroporous resins coupled with preparative chromatographic method

被引：11
|
作者：
Zhou, Zhengzheng [1 ]
Luo, Jianguang [1 ]
Wang, Junsong [1 ]
Li, Liang [1 ]
Kong, Lingyi [1 ]
机构：
[1] China Pharmaceut Univ, Dept Nat Med Chem, Nanjing 210009, Jiangsu, Peoples R China
来源：
NATURAL PRODUCT RESEARCH | 2015年 / 29卷 / 02期
基金：
中国国家自然科学基金;
关键词：
epimedin C; icariin; epimedin B; separation; Epimedium; epimedin A; COUNTER-CURRENT CHROMATOGRAPHY; PURIFICATION; EXTRACTS; ICARIIN;
D O I：
10.1080/14786419.2014.964704
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
An efficient, feasible enrichment and separation method of epimedins A, B, C and icariin from Herba Epimedii was developed by the combination of microwave-assisted extraction, macroporous resins and preparative HPLC. WDX-5 macroporous resin shows better recoveries at 96.2%, 97.0%, 98.2% and 97.1% for epimedins A, B, C and icariin than other macroporous resins used in the experiments. As a result, epimedins A (5.1mg), B (15.3mg), C (7.6mg) and icariin (14.3mg) were obtained from 6.0g crude Herba Epimedii with the recoveries at 70.8%, 68.9%, 66.7% and 95.3%, respectively. The method developed in this study may provide scientific references for the enrichment and separation of flavonoids from Herba Epimedii.
引用
收藏
页码：185 / 188
页数：4
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